Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,268,596 T→C 50.0% T12A (ACT→GCT)  yeiW ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,268,5960TC50.0% ‑6.1 / 16.9 14T12A (ACT→GCT) yeiWconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (3/4);  new base C (2/5);  total (5/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.65e-01

TGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGAT  >  W3110S.gb/2268530‑2268663
                                                                  |                                                                   
tGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCaa                                                                 <  1:1461621/71‑1 (MQ=255)
       cGTATTGGCGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGccc                                                          <  1:740740/71‑1 (MQ=255)
           ttGGCGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGc                                                       >  1:1181153/1‑70 (MQ=255)
           ttGGCGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGc                                                       >  1:1530375/1‑70 (MQ=255)
           ttGGCGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGc                                                       >  1:2258717/1‑70 (MQ=255)
           ttGGCGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGc                                                       >  1:932474/1‑70 (MQ=255)
                gggCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCg                                                 <  1:121609/71‑1 (MQ=255)
                                   ccGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACgtt                               <  1:395796/70‑1 (MQ=255)
                                          tAGGGCTGGAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTtctc                         >  1:854456/1‑69 (MQ=255)
                                                  gAAATTGAAGGGGCAGCGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTa                <  1:765132/70‑1 (MQ=255)
                                                      ttGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAacca            <  1:2635618/70‑1 (MQ=255)
                                                      ttGAAGGGGCAGCGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAg                    <  1:2229040/62‑1 (MQ=255)
                                                         aaGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCg         <  1:2398281/70‑1 (MQ=255)
                                                               cAGCGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATtgat  <  1:1371094/71‑1 (MQ=255)
                                                                  |                                                                   
TGCAGGGCGTATTGGCGGGCTTGCCATCTGGCATACCGGGAATAGGGCTGGAAATTGAAGGGGCAGTGCAACACGCCCCGCAACCCGGACGGCATTCCATACGTTGTTCTCTTAAGGTTAACCACCGGATTGAT  >  W3110S.gb/2268530‑2268663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑