| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,271,945 | A→G | 42.5% | R261R (CGA→CGG) | yeiR → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,271,945 | 0 | A | G | 42.5% | 12.6 / 50.0 | 40 | R261R (CGA→CGG) | yeiR | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (6/17); new base G (5/12); total (11/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01 | |||||||||||
CAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGTGCGAATCAACCGT > W3110S.gb/2271877‑2272011 | cAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGagt > 1:11110/1‑69 (MQ=255) aGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATTGGCGCGACtt > 1:291796/1‑71 (MQ=255) aGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACtt > 1:170100/1‑71 (MQ=255) ccTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCa > 1:762856/1‑71 (MQ=255) cGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCAcc < 1:972875/69‑1 (MQ=255) cGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCAcc < 1:969594/69‑1 (MQ=255) cGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCAcc < 1:2585530/69‑1 (MQ=255) cTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGGCTTGCACCGGTgg < 1:1489563/71‑1 (MQ=255) cTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGGCTTGCACCGGTgg < 1:1592782/71‑1 (MQ=255) cTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGGCTTGCACCGGTgg < 1:2176405/71‑1 (MQ=255) ggATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGGCTTGCACCGGTGGaa > 1:718518/1‑71 (MQ=255) gACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCa < 1:1322870/70‑1 (MQ=255) gACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCa < 1:2664558/70‑1 (MQ=255) cGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAg < 1:2441373/71‑1 (MQ=255) gCTGATAGGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGGCTTGc < 1:18093/50‑1 (MQ=39) cTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGGCTTGCACCGGTGGAACGCGTCa < 1:1650487/66‑1 (MQ=255) cTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGGCTTGCACCGGTGGAACGCGTCa < 1:330441/66‑1 (MQ=255) cTGATACGGTATTCCACACCATTGGCATTCTGGAATGGGCGCGGCTTGCACCGGTGGAACGCGTCa < 1:2693169/66‑1 (MQ=255) aCGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGtgct > 1:900327/1‑71 (MQ=255) cGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGGCTTGCAcc > 1:1157850/1‑46 (MQ=255) cGGTATTCGACACCATTGGCATTCTGGAATGGGAGCGGCTTGCAcc > 1:2321116/1‑46 (MQ=38) gTATTCGACACCATTGGCATTCTGGAATGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCg < 1:1905492/71‑1 (MQ=255) gTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCg < 1:1026946/71‑1 (MQ=255) tATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGt < 1:2205049/71‑1 (MQ=255) ttCGACACCATTGGCATTCTGGAATGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGtg > 1:345464/1‑63 (MQ=255) ttCGACACCATTGGCATTCTGGAATGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTAt < 1:961269/71‑1 (MQ=255) acacCATTGGCATTCTGGAATGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCg < 1:2113930/64‑1 (MQ=255) acCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGa < 1:240559/71‑1 (MQ=255) ccATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTgg < 1:362952/39‑1 (MQ=255) ccATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTgg < 1:1204761/39‑1 (MQ=255) cATTGGCATTCTGGAATGGGCGCGGCTTGCACCGGTGGAACGCGTCaaa < 1:1651500/49‑1 (MQ=255) aTTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGt < 1:2686482/53‑1 (MQ=255) aTTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGt < 1:431646/53‑1 (MQ=255) ttCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCg < 1:328502/52‑1 (MQ=255) cTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGTGc > 1:1041107/1‑70 (MQ=255) gAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGTGCGa < 1:727938/69‑1 (MQ=255) gAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGTGCGa < 1:1741551/69‑1 (MQ=255) tGGGCGCGGCTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAg < 1:409136/55‑1 (MQ=255) tGGGCGCGACTTGCACCGGTCGAACGCGTCAAAGGCGTGCTGCg < 1:2393947/44‑1 (MQ=255) ggCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGTGCGAATCAAc > 1:2657098/1‑70 (MQ=255) gcgACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGTGCGAATCAACCGt > 1:496176/1‑70 (MQ=255) | CAGGCCTGCGGCTGGATATTCGACGCTGATACGGTATTCGACACCATTGGCATTCTGGAATGGGCGCGACTTGCACCGGTGGAACGCGTCAAAGGCGTGCTGCGTATTCCCGAAGGGCTGGTGCGAATCAACCGT > W3110S.gb/2271877‑2272011 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |