| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,274,249 | A→G | 100% | T64A (ACT→GCT) | rtn → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,274,249 | 0 | A | G | 85.7% | 89.7 / 7.3 | 35 | T64A (ACT→GCT) | rtn | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (4/1); new base G (14/16); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AATACGACACACTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCTAACCCC > W3110S.gb/2274184‑2274302 | aaTACGACACACTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCgctact < 1:260167/71‑1 (MQ=255) tACGACACACTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGa > 1:2041847/1‑71 (MQ=255) cTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCa < 1:142273/71‑1 (MQ=255) cTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCa < 1:2259543/71‑1 (MQ=255) cTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGATc > 1:228043/1‑69 (MQ=255) aTTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGc > 1:2481417/1‑70 (MQ=255) ttACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCg > 1:2584946/1‑71 (MQ=255) gACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGAc < 1:2705625/71‑1 (MQ=255) gACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGAc < 1:2241724/71‑1 (MQ=255) tACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTa < 1:2687938/71‑1 (MQ=255) tACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTa < 1:2786241/71‑1 (MQ=255) tACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTa < 1:461205/71‑1 (MQ=255) tACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTa < 1:1202206/71‑1 (MQ=255) aaaGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATa < 1:2317546/71‑1 (MQ=255) aaaGTATCTCGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATa > 1:801658/1‑71 (MQ=255) tctcGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGa < 1:2450105/56‑1 (MQ=255) tctcGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGa < 1:1544726/56‑1 (MQ=255) tctcGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGAt < 1:2504505/64‑1 (MQ=255) tcGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCa > 1:867761/1‑70 (MQ=255) tcGATACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAg > 1:65633/1‑71 (MQ=255) tcGATACCTATTTTGCCGACCTGAAATCCACTACTGAc > 1:1754069/1‑38 (MQ=255) tcGATACCTATTTTGCCGACCTGAAATCCACTACTGAc > 1:1689348/1‑38 (MQ=255) tcGATACCTATTTTGCCGACCTGAAATCCACTACTGAc > 1:2699789/1‑38 (MQ=255) aTACCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTa < 1:2516789/56‑1 (MQ=255) aCCTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGa > 1:1247740/1‑56 (MQ=255) ccTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCt < 1:2413481/71‑1 (MQ=255) ccTATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCt < 1:22095/71‑1 (MQ=255) tATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCa < 1:657502/65‑1 (MQ=255) tATTTTGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCTaa > 1:39929/1‑71 (MQ=255) ttttGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCt < 1:1572438/67‑1 (MQ=255) ttttGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCTAAcc > 1:843284/1‑71 (MQ=255) tttGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGc > 1:2676656/1‑40 (MQ=255) ttGCCGACCTGAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCTAAcccc > 1:1578765/1‑71 (MQ=255) gAAATCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGa > 1:156973/1‑39 (MQ=255) aaaTCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGa > 1:2456472/1‑38 (MQ=255) aaaTCCGCTACTGACCGGCTCCAGCCGCTGACCTTAGa > 1:1591396/1‑38 (MQ=255) | AATACGACACACTGATTACCGACGTACAAAAGTATCTCGATACCTATTTTGCCGACCTGAAATCCACTACTGACCGGCTCCAGCCGCTGACCTTAGATACCTGCCAGCAAGCTAACCCC > W3110S.gb/2274184‑2274302 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |