| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,291,258 | C→T | 9.2% | G326D (GGC→GAC) | yejO ← | predicted autotransporter outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,291,258 | 0 | C | T | 9.2% | 159.1 / 5.5 | 54 | G326D (GGC→GAC) | yejO | predicted autotransporter outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (23/26); new base T (2/3); total (25/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
TAATTTATTTGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGT > W3110S.gb/2291189‑2291325 | tAATTTATTTGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGc > 1:725707/1‑70 (MQ=255) tttGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCa < 1:648162/70‑1 (MQ=255) cGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGt < 1:2219975/71‑1 (MQ=255) tCATGACCTCAAGGATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAgcg > 1:41466/1‑71 (MQ=255) tCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGcc > 1:1805831/1‑59 (MQ=255) tCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGc > 1:2432877/1‑58 (MQ=255) tGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGc > 1:2168924/1‑51 (MQ=255) aCCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGc > 1:2032711/1‑70 (MQ=255) aCCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGc < 1:2311375/70‑1 (MQ=255) cTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAt < 1:2718050/71‑1 (MQ=255) cTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAt < 1:442024/71‑1 (MQ=255) cAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGcc > 1:1612794/1‑50 (MQ=255) cAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGcc > 1:313546/1‑50 (MQ=255) cAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttg > 1:2561256/1‑71 (MQ=255) cAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAttg > 1:951454/1‑71 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAg > 1:412094/1‑47 (MQ=255) aaGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAtt < 1:982168/69‑1 (MQ=255) tGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAg < 1:2222739/54‑1 (MQ=255) gCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGa > 1:2115879/1‑47 (MQ=255) ccATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGt < 1:41568/49‑1 (MQ=255) ccATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGt < 1:1799191/49‑1 (MQ=255) catcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCa < 1:1092334/43‑1 (MQ=255) tcatTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGtt < 1:2114305/47‑1 (MQ=255) tcatTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGtt < 1:1715278/47‑1 (MQ=255) tcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAAcc < 1:2520321/69‑1 (MQ=255) tcatTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCat < 1:2046574/71‑1 (MQ=255) tcatTACCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCa > 1:2027812/1‑70 (MQ=255) atTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCatat > 1:940439/1‑71 (MQ=255) atTCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCatat > 1:273648/1‑71 (MQ=255) atTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGa > 1:870360/1‑41 (MQ=255) atTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGa > 1:2683160/1‑41 (MQ=255) ttCCCTCTGACTGAAACGTTAACCATTGTGTCAGCCCAGACGTTAGCGCGGCCAttg < 1:1473950/57‑1 (MQ=255) ttCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAtt < 1:681638/56‑1 (MQ=255) ttCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCAtt < 1:1232020/56‑1 (MQ=255) cccTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATAttt > 1:464107/1‑70 (MQ=255) actgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:358649/68‑1 (MQ=255) actgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:1261987/68‑1 (MQ=255) actgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga < 1:1610278/68‑1 (MQ=255) actgaAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGAt > 1:1155654/1‑71 (MQ=255) cGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCtttt > 1:358324/1‑71 (MQ=255) gTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTg < 1:367615/71‑1 (MQ=255) gTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTg < 1:612519/71‑1 (MQ=255) ttAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATa > 1:390301/1‑45 (MQ=255) ttAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATa > 1:2128822/1‑45 (MQ=255) ttAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCa < 1:838718/57‑1 (MQ=255) aaCCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga > 1:606246/1‑57 (MQ=255) aaCCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga > 1:1243433/1‑57 (MQ=255) aaCCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCaga > 1:412515/1‑57 (MQ=255) aCCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAgg < 1:1595116/70‑1 (MQ=255) aCCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAgg < 1:224436/70‑1 (MQ=255) ccATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGaa < 1:2131132/71‑1 (MQ=255) aTTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATa < 1:1539336/71‑1 (MQ=255) ttGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCa < 1:1047314/42‑1 (MQ=255) gtgCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGt > 1:218795/1‑71 (MQ=255) gtgCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATATTCAGAGATGGCATTTGAGGAATACg > 1:1070686/1‑70 (MQ=255) tgCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGt < 1:839154/70‑1 (MQ=255) | TAATTTATTTGCGGCTTCATGACCTCAAGAATGCCATCATTCCCTCTGACTGAAACGTTAACCATTGTGCCAGCCCAGACGTTAGCGCGGCCATTGTTGATAACCATATTTTCAGAGATGGCTTTTGAGGAATACGT > W3110S.gb/2291189‑2291325 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |