| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,298,568 | T→C | 32.0% | E49E (GAA→GAG) | ccmE ← | periplasmic heme chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,298,568 | 0 | T | C | 32.0% | 25.1 / 19.5 | 25 | E49E (GAA→GAG) | ccmE | periplasmic heme chaperone |
| Reads supporting (aligned to +/- strand): ref base T (11/6); new base C (6/2); total (17/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
CACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGAGCGCAGCGCA > W3110S.gb/2298504‑2298631 | cacTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGc > 1:1225205/1‑71 (MQ=255) cTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCtt < 1:1467238/71‑1 (MQ=255) cTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCtt < 1:1470520/71‑1 (MQ=255) aCCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGc > 1:2600231/1‑71 (MQ=255) ggCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCg > 1:2145315/1‑69 (MQ=255) aTCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTa > 1:448326/1‑68 (MQ=255) aCCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGaa > 1:2600469/1‑70 (MQ=255) aCCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAAt > 1:641844/1‑71 (MQ=255) aCCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAAt > 1:2597168/1‑71 (MQ=255) aTCCCGCCAACGCGCAGCCGCTGACCGACTTCCGGCATTTGCTGAGTTTCa > 1:233336/1‑51 (MQ=255) ccGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTccc > 1:2453525/1‑70 (MQ=255) ccAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGaa < 1:2652158/61‑1 (MQ=255) aaCGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCg > 1:2370507/1‑71 (MQ=255) gACTTCCGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGatat > 1:640159/1‑71 (MQ=255) ccGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCg < 1:201283/69‑1 (MQ=255) ccGGCATTTGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCg < 1:2600412/69‑1 (MQ=255) cGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGag < 1:2734008/70‑1 (MQ=255) tGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcagcg > 1:2470509/1‑70 (MQ=255) tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcagcg > 1:2111564/1‑70 (MQ=255) tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcagcg > 1:2617830/1‑70 (MQ=255) tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcagcg > 1:2707315/1‑70 (MQ=255) tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcagcg < 1:323543/70‑1 (MQ=255) tGCTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGagcgcagcg > 1:1037747/1‑70 (MQ=255) cTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGAgcgcagcgca < 1:2226251/70‑1 (MQ=255) cTGAGTCTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGAgcgcagcgca > 1:1390722/1‑70 (MQ=255) | CACTACCCGGCATCACCATCCCGCCAACGCGCAGACGCTGACCGACTTCCGGCATTTGCTGAGTTTCACGCTTGCCGTAGAGAATTTCCCCCGGCGTATAAAAGAGATCGATATTCGAGCGCAGCGCA > W3110S.gb/2298504‑2298631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |