| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,307,310 | C→T | 77.8% | V24V (GTC→GTT) | eco → | ecotin, a serine protease inhibitor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,307,310 | 0 | C | T | 77.8% | 48.0 / 12.1 | 27 | V24V (GTC→GTT) | eco | ecotin, a serine protease inhibitor |
| Reads supporting (aligned to +/- strand): ref base C (4/2); new base T (7/14); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01 | |||||||||||
GACCATTCTACCTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGATTCAG > W3110S.gb/2307244‑2307376 | gACCATTCTACCTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGc < 1:1382767/71‑1 (MQ=255) ccATTCTACCTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCa < 1:1206034/71‑1 (MQ=255) aGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAAt < 1:2077152/69‑1 (MQ=255) ttGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGcc > 1:1546882/1‑71 (MQ=255) ttGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGcc > 1:733888/1‑71 (MQ=255) ttGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGaaaaa < 1:2098556/63‑1 (MQ=255) gTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCt > 1:12417/1‑70 (MQ=255) tttGCCGCTTTCGCTACCACTTCCGCCTTGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCtt < 1:1295096/70‑1 (MQ=255) tttGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGaa < 1:317805/57‑1 (MQ=255) tttGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCtt < 1:1542188/70‑1 (MQ=255) tttGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCtt < 1:2329667/70‑1 (MQ=255) tGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATc > 1:1680457/1‑71 (MQ=255) cTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACaa < 1:495299/71‑1 (MQ=255) cTACCACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGaa < 1:2469568/71‑1 (MQ=255) ccACTTCCGCCTGGGCGGCAGAAAGCGTTCAGCCACTGGAAAAAATcgcg < 1:2391455/50‑1 (MQ=255) tGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCg > 1:1500515/1‑71 (MQ=255) ggcggcAGAAAGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTc < 1:1474730/71‑1 (MQ=255) gcAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCtt < 1:1960251/37‑1 (MQ=255) aGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGa > 1:1905181/1‑71 (MQ=255) aaaGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAgg < 1:2172572/52‑1 (MQ=255) aaaGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAgg < 1:1007251/52‑1 (MQ=255) aaaGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAgg < 1:2485057/52‑1 (MQ=255) aGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGaaaaa > 1:285529/1‑48 (MQ=255) aGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAg > 1:188977/1‑63 (MQ=255) aGCGTTCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGAtt > 1:347738/1‑69 (MQ=255) aGCGTCCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGAtt > 1:1470553/1‑69 (MQ=255) gCGTCCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGATTCAg > 1:818700/1‑71 (MQ=255) | GACCATTCTACCTGCAGTATTGTTTGCCGCTTTCGCTACCACTTCCGCCTGGGCGGCAGAAAGCGTCCAGCCACTGGAAAAAATCGCGCCTTATCCACAAGCTGAAAAAGGGATGAAGCGTCAGGTGATTCAG > W3110S.gb/2307244‑2307376 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |