| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,335,995 | C→T | 21.9% | pseudogene (3078/4605 nt) pseudogene (3078/4104 nt) |
yfaS ← yfaS ← |
ECK2220:JW2222+JW2221:b4500; hypothetical protein ECK2220:JW2222:b2228; hypothetical protein, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,335,995 | 0 | C | T | 21.9% | 64.5 / 13.8 | 32 | pseudogene (3078/4605 nt) pseudogene (3078/4104 nt) | yfaS yfaS | ECK2220:JW2222+JW2221:b4500; hypothetical protein ECK2220:JW2222:b2228; hypothetical protein, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base C (20/5); new base T (3/4); total (23/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.64e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
TCACTACTTTGTAGCCGGATATTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCTAATGCTGT > W3110S.gb/2335927‑2336062 | tCACTACTTTGTAGCCGGATATTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCAt > 1:590533/1‑71 (MQ=255) actactTTGTAGCCGGATATTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGTATGa > 1:1140610/1‑71 (MQ=255) atTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGTATGACATTTTTCTTCTGTTc < 1:362603/69‑1 (MQ=255) atTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCaa > 1:1734834/1‑71 (MQ=255) ttGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGTATGACATTTTTCTGCTGTTc < 1:2174786/68‑1 (MQ=255) ttGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGTATGACATTTTTCTGCTGTTc < 1:60908/68‑1 (MQ=255) ttGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAAc > 1:1237497/1‑71 (MQ=255) tGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCaa > 1:1209894/1‑69 (MQ=255) gCTCGGCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGTATGACATTTTTctgc > 1:1736067/1‑61 (MQ=255) gCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACgg > 1:2752998/1‑71 (MQ=255) gCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACgg > 1:144327/1‑71 (MQ=255) cTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGTATGACATTTTTCTGCTGTTCAACgg < 1:380001/70‑1 (MQ=255) tGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACgg < 1:872644/64‑1 (MQ=255) ggCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATc > 1:1925949/1‑71 (MQ=255) cATCAGCGCGTTATCGCCACCACCGAGCATGATATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCcaca > 1:2623125/1‑70 (MQ=255) aTCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCaa > 1:2627200/1‑49 (MQ=255) aTCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACaa > 1:676540/1‑70 (MQ=255) aGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCaa > 1:67286/1‑46 (MQ=255) aGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCaa > 1:796301/1‑46 (MQ=255) aGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGa > 1:1031309/1‑71 (MQ=255) gcgcGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGAc < 1:1585039/71‑1 (MQ=255) aTCGCCACCACCGAGTATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTtg > 1:2409432/1‑71 (MQ=255) aTCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAAc > 1:1178255/1‑39 (MQ=255) aTCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATc < 1:1096261/54‑1 (MQ=255) aTCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTtg > 1:2009063/1‑71 (MQ=255) aTCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTtg > 1:1697181/1‑71 (MQ=255) gCCACCACCGAGCATGACATTTTTCTGCTGTTCaa > 1:1173232/1‑35 (MQ=255) gCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGc > 1:974684/1‑40 (MQ=255) ccaccaCCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCt < 1:1551733/71‑1 (MQ=255) ccaccGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCTAAt < 1:321181/71‑1 (MQ=255) gAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCTAATGCTGt > 1:671739/1‑71 (MQ=255) gCATGACATTTTTCTGCTGTTCAACGGGCCAGGTGTTAt > 1:157805/1‑39 (MQ=255) | TCACTACTTTGTAGCCGGATATTGCTCGCCTGCTCGGGCAACATCAGCGCGTTATCGCCACCACCGAGCATGACATTTTTCTGCTGTTCAACGGGCCAGCTGTTATCCACAAAAGACAGTTTTGTGCTAATGCTGT > W3110S.gb/2335927‑2336062 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |