| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,345,374 | C→T | 24.3% | G1156S (GGT→AGT) | yfaL ← | adhesin |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,345,374 | 0 | C | T | 24.3% | 69.0 / 19.1 | 37 | G1156S (GGT→AGT) | yfaL | adhesin |
| Reads supporting (aligned to +/- strand): ref base C (15/13); new base T (6/3); total (21/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCA > W3110S.gb/2345305‑2345442 | aTGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCAccc > 1:2378279/1‑71 (MQ=255)aTGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCAcc < 1:1092769/70‑1 (MQ=255) ttGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGc > 1:626795/1‑71 (MQ=255) tGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACTCTGCGATtgt < 1:1752630/71‑1 (MQ=255) aCATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCAcc > 1:2576155/1‑59 (MQ=255) aCATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCAcc > 1:1094963/1‑59 (MQ=255) aCATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGa > 1:2478508/1‑71 (MQ=255) aCATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGa < 1:1305433/71‑1 (MQ=255) aCATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGa > 1:2319025/1‑71 (MQ=255) cATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGac < 1:668911/71‑1 (MQ=255) aTGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGa > 1:2552381/1‑69 (MQ=255) aaCAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACTCTGCGATTGTGACAcgc > 1:1208650/1‑71 (MQ=255) cAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACTCTGCGATTGTGCCAcgcg > 1:2181742/1‑70 (MQ=255) aGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACTCTGCGATTGTGACAcgcgcg < 1:1019541/71‑1 (MQ=255) cGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTa > 1:1908708/1‑65 (MQ=255) cGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAgcggc < 1:2772578/70‑1 (MQ=255) tgtAAACCCAGCCGCGTCTGAATATCATCACTCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTaa < 1:662788/70‑1 (MQ=255) tgtAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTa < 1:837380/69‑1 (MQ=255) aaCCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAatca > 1:2165466/1‑71 (MQ=255) aaCCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAatca > 1:1871424/1‑71 (MQ=255) aaCCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAatca < 1:2477416/71‑1 (MQ=255) aCCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAatc < 1:1293929/69‑1 (MQ=255) ccAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATcc > 1:1887032/1‑71 (MQ=255) ccAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATcc > 1:1886221/1‑71 (MQ=255) ccAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATcc > 1:2391376/1‑71 (MQ=255) gCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAatc < 1:2544076/64‑1 (MQ=255) cgTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAat < 1:748557/59‑1 (MQ=255) cgTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAat < 1:802224/59‑1 (MQ=255) tCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACg > 1:2591025/1‑71 (MQ=255) cTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCctgc > 1:1137078/1‑64 (MQ=255) aaTATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAatca > 1:1044313/1‑55 (MQ=255) tcatcaCTCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGccc > 1:1157036/1‑66 (MQ=255) tcatcaCCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAatc < 1:1870173/50‑1 (MQ=255) tcatcaCCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAatc < 1:1826269/50‑1 (MQ=255) tcatcaCCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCctgctg < 1:2522099/59‑1 (MQ=255) atcaCTCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAatcatc > 1:2556034/1‑51 (MQ=255) atcaCTCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAatcatc > 1:743069/1‑51 (MQ=255) atcaCTCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAatcatc > 1:965554/1‑51 (MQ=255) atcaCCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAatcat > 1:2647684/1‑50 (MQ=255) tcaCCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAatca < 1:2694252/48‑1 (MQ=255) aCCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCa > 1:1051115/1‑71 (MQ=255) | ATGTTGGTATGACATGAACAGCGGTACGCCATTCGCTGTGTAAACCCAGCCGCGTCTGAATATCATCACCCTGCGATTGTGACACGCGCGCACGGTTAGCGGCGGTAAAATCATCCTGCTGCACGCCCTGATAAATCA > W3110S.gb/2345305‑2345442 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |