| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,426,403 | G→A | 70.4% | G113R (GGG→AGG) | yfcG → | predicted glutathione S‑transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,426,403 | 0 | G | A | 70.4% | 35.6 / 17.5 | 27 | G113R (GGG→AGG) | yfcG | predicted glutathione S‑transferase |
| Reads supporting (aligned to +/- strand): ref base G (2/6); new base A (15/4); total (17/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.48e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGG > W3110S.gb/2426344‑2426469 | aGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAatcat > 1:2582653/1‑71 (MQ=255)aGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAatcat > 1:196776/1‑71 (MQ=255) caTTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAAt < 1:760501/68‑1 (MQ=255) ttACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCAc > 1:2097868/1‑69 (MQ=255) ttACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACg > 1:842787/1‑70 (MQ=255) tACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATAATTTTAATCACg > 1:1883957/1‑69 (MQ=255) gTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGccc > 1:1066106/1‑71 (MQ=255) tGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGcccc > 1:615141/1‑71 (MQ=255) gTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACg > 1:1937076/1‑62 (MQ=255) gTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGccccc > 1:2005636/1‑70 (MQ=255) aGGTAGGCGGACTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCt < 1:2014506/71‑1 (MQ=255) ggTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAAt < 1:1299611/46‑1 (MQ=255) gTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTa > 1:743924/1‑71 (MQ=255) ggCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGc < 1:186690/71‑1 (MQ=255) gCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCt < 1:2506168/71‑1 (MQ=255) gACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCAtt < 1:230049/59‑1 (MQ=255) aCTGGGGCCGATGCTTGGGCAAAATAATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTg < 1:1958557/71‑1 (MQ=255) cTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGa < 1:466514/71‑1 (MQ=255) cTGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGccgccc > 1:380878/1‑49 (MQ=255) tGGGGCCGATGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGaa > 1:2090003/1‑71 (MQ=255) aTGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGt < 1:1792223/66‑1 (MQ=255) tGCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTg < 1:1604025/60‑1 (MQ=255) gCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTa > 1:1070316/1‑66 (MQ=255) gCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTa > 1:1067080/1‑66 (MQ=255) gCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAgg > 1:2630058/1‑71 (MQ=255) gCTTAGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAgg > 1:2629185/1‑71 (MQ=255) cTTAGGCAAAATCATCATTTTAATCACGCAGcccc > 1:2626803/1‑35 (MQ=255) | AGCGCGCCGCCACATTACAGTGGTTATTCTGGCAGGTAGGCGGACTGGGGCCGATGCTTGGGCAAAATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGG > W3110S.gb/2426344‑2426469 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |