| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,440,837 | C→T | 64.5% | A82A (GCG→GCA) | truA ← | pseudouridylate synthase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,440,837 | 0 | C | T | 64.5% | 25.6 / 28.5 | 31 | A82A (GCG→GCA) | truA | pseudouridylate synthase I |
| Reads supporting (aligned to +/- strand): ref base C (5/6); new base T (12/8); total (17/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTG > W3110S.gb/2440777‑2440893 | aGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCATGCCGCGTCtt < 1:2010422/71‑1 (MQ=255) ttAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTgcg > 1:942213/1‑71 (MQ=255) gCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCATGCCGCGTCtt > 1:835932/1‑57 (MQ=255) acaGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGCGCGgttgt < 1:2486862/71‑1 (MQ=255) cGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCATGCCGCGTCTTTgcg > 1:2316297/1‑55 (MQ=255) gATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGa < 1:1202166/71‑1 (MQ=255) gATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGa < 1:463173/71‑1 (MQ=255) gATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGa < 1:461567/71‑1 (MQ=255) gATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGa < 1:2258954/71‑1 (MQ=255) gTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAAt > 1:1282747/1‑71 (MQ=255) gTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAAt > 1:850739/1‑71 (MQ=255) tCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATg > 1:1754756/1‑71 (MQ=255) cAGGTAAATTCGCATTTACGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGcaca < 1:180943/71‑1 (MQ=255) cAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTc < 1:1082057/40‑1 (MQ=255) aGGTAAATTCGCATTTACGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGcaca < 1:582423/70‑1 (MQ=255) aGGTAAATTCGCATTTACGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACaa < 1:2186822/71‑1 (MQ=255) ggTAAATTCGCATTTACGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAAc > 1:873729/1‑71 (MQ=255) ggTAAATTCGCATTTACGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAAc > 1:266936/1‑71 (MQ=255) ggTAAATTCGCATTTACGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAAc > 1:1552384/1‑71 (MQ=255) ggTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCtt > 1:1930556/1‑40 (MQ=255) ggTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAAc > 1:2287959/1‑71 (MQ=255) gTAAATTCGCATTTACGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAAcc > 1:2176665/1‑71 (MQ=255) gTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAAc > 1:1012963/1‑70 (MQ=255) aaaTTCGCATTTACGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGaaa > 1:218048/1‑60 (MQ=255) gCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGt < 1:1457762/71‑1 (MQ=255) tttACGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGcac < 1:490408/56‑1 (MQ=255) tttACGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACaa < 1:587654/58‑1 (MQ=255) tttACGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTAcc > 1:2687356/1‑71 (MQ=255) aCGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGcac > 1:1823781/1‑53 (MQ=255) aCGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCgtg > 1:1824405/1‑71 (MQ=255) cGCCCAAGGTCCATGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCgtg < 1:1842026/70‑1 (MQ=255) | AGTTTTAACCCAACGCACAGCGATGTCACCAGGTAAATTCGCATTTACGCCCAAGGTCCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTG > W3110S.gb/2440777‑2440893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |