| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,452,377 | C→T | 72.0% | P181P (CCG→CCA) | aroC ← | chorismate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,452,377 | 0 | C | T | 72.0% | 33.8 / 15.5 | 25 | P181P (CCG→CCA) | aroC | chorismate synthase |
| Reads supporting (aligned to +/- strand): ref base C (2/5); new base T (9/9); total (11/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
AGTCGCCCTCTTTTTTCAGCGCACGCATCAACTCGTCTAACGCGTCGATTTTGTCGGGGTCCGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCTTTGATATCCAGCGGAATGTCGCCCATCTGGG > W3110S.gb/2452316‑2452447 | aGTCGCCCTCTTTTTTCAGCGCACGCATCAACTCGTCTAACGCGTCGATTTTGTCGGGGTCCGGGCaaaaa < 1:2592039/71‑1 (MQ=255) ttttttCAGCGCACGCATCAACTCGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGAtttt > 1:188290/1‑71 (MQ=255) tttCAGCGCACGCATCAACTCGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTg < 1:2390508/69‑1 (MQ=255) tttCAGCGCACGCATCAACTCGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGc < 1:1134119/70‑1 (MQ=255) cacgcaTCCACCCGTCTAACGCGGCGATTTTGTCGGGGGCTGGGCAAAAAAACGGATTTTGCTCg < 1:1569943/65‑1 (MQ=255) tCAACTCGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGc < 1:66779/56‑1 (MQ=255) aaCTCGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGAtt > 1:1942381/1‑50 (MQ=255) aaCTCGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc > 1:2339601/1‑71 (MQ=255) aaCTCGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc > 1:2534306/1‑71 (MQ=255) aaCTCGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc > 1:1821416/1‑71 (MQ=255) aaCTCGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTc > 1:1818075/1‑71 (MQ=255) aaCTCGTCTAACGCGTCGATTTTGTCGGGGTCCGGGCAAAAAAACGGATTTTGCTCGa < 1:2186671/58‑1 (MQ=255) aaCTCGTCTAACGCGTCGATTTTGTCGGGGTCCGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGt > 1:1651055/1‑70 (MQ=255) cGTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTg < 1:2111556/49‑1 (MQ=255) gTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCttt < 1:2680227/69‑1 (MQ=255) gTCTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCTTTGa > 1:2641320/1‑71 (MQ=255) cTAACGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCTTTGata > 1:1043379/1‑71 (MQ=255) cTAACGCGTCGATTTTGTCGGGGTCCGGGCAAAAAAACGGAttt > 1:2600639/1‑44 (MQ=255) tAACGCGTCGATTTTGTCGGGGTCCGGGCaaaaaa < 1:820742/35‑1 (MQ=255) aCGCGTCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCttt < 1:2185611/64‑1 (MQ=255) tCGATTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCTTTGATATCCAGCgg < 1:1433858/71‑1 (MQ=255) tCGATTTTGTCGGGGTCCGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCTTTGATATCCAGCgg < 1:494968/71‑1 (MQ=255) aTTTTGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCTTTGATATCCAGCgg < 1:2271927/68‑1 (MQ=255) tttGTCGGGGTCTGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCTTTGATATCCAGCGGAATGt > 1:815709/1‑71 (MQ=255) tGTCGGGGTCCGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCTTTGATATCCAGCGGAATGTc < 1:2644845/70‑1 (MQ=255) cGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCTTTGATATCCAGCGGAATGTCGCCCATCTggg > 1:2484417/1‑71 (MQ=255) | AGTCGCCCTCTTTTTTCAGCGCACGCATCAACTCGTCTAACGCGTCGATTTTGTCGGGGTCCGGGCAAAAAAACGGATTTTGCTCGACCTGCGACCAGTCTTTGATATCCAGCGGAATGTCGCCCATCTGGG > W3110S.gb/2452316‑2452447 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |