Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,512,230 A→G 100% W371R (TGG→CGG)  ypdG ← predicted enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,512,2300AG92.9% 36.5 / ‑4.3 14W371R (TGG→CGG) ypdGpredicted enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (7/6);  total (7/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAGC  >  W3110S.gb/2512166‑2512296
                                                                |                                                                  
aTGACTGCCCCCAGCGCGCTCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGc                                                              >  1:1493956/1‑71 (MQ=255)
aTGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGc                                                              >  1:1496190/1‑71 (MQ=255)
aTGACTCCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGc                                                              >  1:3338/1‑71 (MQ=255)
   aCTGCCCCCAGCGCGATCCCCGCCTTATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGa                                                           <  1:954641/71‑1 (MQ=255)
               gcgATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAAcc                                                <  1:1907279/70‑1 (MQ=255)
                cgATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCAt                                              <  1:2790783/71‑1 (MQ=255)
                   tCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGc                                           <  1:2467037/71‑1 (MQ=255)
                   tCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGc                                           <  1:1035519/71‑1 (MQ=255)
                    ccccGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCa                                          >  1:385363/1‑71 (MQ=255)
                    ccccGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCa                                          >  1:599754/1‑71 (MQ=255)
                            tataGACGCCCAGGTTAGTGACCAGCGGCCATGCCCGGATAGCg                                                             <  1:2350622/44‑1 (MQ=255)
                                    cccAGGTTAGTGACCAGCGGCCATGCCCGGATAGCg                                                             <  1:1352102/36‑1 (MQ=255)
                                           tAGTGACCAGCGGCCATGCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAgcggcg                   >  1:1028183/1‑71 (MQ=255)
                                                            gCCCGGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAgc  >  1:2194024/1‑71 (MQ=255)
                                                                |                                                                  
ATGACTGCCCCCAGCGCGATCCCCGCCATATAGACGCCCAGGTTAGTGACCAGCGGCCATGCCCAGATAGCGGACTCCGGGAACCATTGCACTGCGCCCAGCCAGACAGCGGCGGTTGAGCCGACAATAGC  >  W3110S.gb/2512166‑2512296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑