| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,559,548 | T→C | 60.7% | I293T (ATT→ACT) | hemF → | coproporphyrinogen III oxidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,559,548 | 0 | T | C | 60.7% | 18.0 / 28.7 | 28 | I293T (ATT→ACT) | hemF | coproporphyrinogen III oxidase |
| Reads supporting (aligned to +/- strand): ref base T (5/6); new base C (10/7); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTACGCTGGGAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACG > W3110S.gb/2559483‑2559616 | ggTACGCTGGGAATATGATTATCAGCCAAAAGATGGCAGCCAAGAAGCGGCGTTAAGTGAGTTTACTAAgg > 1:886219/1‑71 (MQ=255) ggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGa < 1:992557/69‑1 (MQ=255) ggAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAG‑TTATTAAGGTCAGGGATTg < 1:2378544/71‑1 (MQ=255) gAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTg < 1:757295/71‑1 (MQ=255) tatGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAgg < 1:1563669/63‑1 (MQ=255) tatGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTgg < 1:2764634/69‑1 (MQ=255) tGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGAtt > 1:679658/1‑65 (MQ=255) tGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTGGgtg > 1:727804/1‑70 (MQ=255) tGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTGGgtg > 1:111099/1‑70 (MQ=255) aaGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCAcccc < 1:2401909/71‑1 (MQ=255) aGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGa > 1:2536020/1‑48 (MQ=255) aTGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTGGGTGTaa > 1:2791190/1‑56 (MQ=255) aTGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTGGGTGTaa > 1:1118333/1‑56 (MQ=255) ggCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTcc > 1:464089/1‑58 (MQ=255) cccAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTGGGTGTAACTCCCt < 1:1275966/55‑1 (MQ=255) cccAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTGGGTGTAACTCCCt < 1:2759680/55‑1 (MQ=255) ccAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCAt > 1:1081618/1‑71 (MQ=255) cAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTGGGTTTAACTcc < 1:1608479/51‑1 (MQ=255) cAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATc > 1:31551/1‑71 (MQ=255) cAGAAGCGGCGTTAAGTGAGTTTACTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATc > 1:1048059/1‑71 (MQ=255) gcggcgTTAAGTGAGTTTACTAAGGTCAGGGATTGGGTGTACCTCCCTCACCCCCACTCCCGCATc > 1:2735917/1‑66 (MQ=255) aaGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCg > 1:2694806/1‑71 (MQ=255) gTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTc < 1:286860/71‑1 (MQ=255) gTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTc < 1:1864856/71‑1 (MQ=255) gAGTTTACTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAg > 1:2191299/1‑71 (MQ=255) gAGTTTACTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAg > 1:2183299/1‑71 (MQ=255) gTTTACTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCa < 1:259615/50‑1 (MQ=255) gTTTACTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCa < 1:1646054/50‑1 (MQ=255) tattaAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACg > 1:790211/1‑71 (MQ=255) tattaAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACg > 1:1376954/1‑71 (MQ=255) | GGTACGCTGGGAATATGATTATCAGCCAAAAGATGGCAGCCCAGAAGCGGCGTTAAGTGAGTTTATTAAGGTCAGGGATTGGGTGTAACTCCCTCACCCCCACTCCCGCATCCGCTGATGCAGCGTCAGTGACG > W3110S.gb/2559483‑2559616 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |