Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 2,560,022 | G→A | 58.3% | P203S (CCT→TCT) | yfeG ← | predicted DNA‑binding transcriptional regulator |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 2,560,022 | 0 | G | A | 58.3% | 6.1 / 27.0 | 24 | P203S (CCT→TCT) | yfeG | predicted DNA‑binding transcriptional regulator |
Reads supporting (aligned to +/- strand): ref base G (5/5); new base A (5/9); total (10/14) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.78e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 |
CTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACAAAGCCCCACAGCGC > W3110S.gb/2559962‑2560088 | cttcCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGga < 1:2201457/69‑1 (MQ=255) tGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGa < 1:908946/71‑1 (MQ=255) tCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCaa < 1:1837754/71‑1 (MQ=255) tCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCaa < 1:1840981/71‑1 (MQ=255) aTTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGt > 1:1245345/1‑71 (MQ=255) ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCa < 1:1081747/64‑1 (MQ=255) ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTg < 1:2346482/40‑1 (MQ=255) ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTg < 1:625695/40‑1 (MQ=255) aGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGAtt > 1:654159/1‑47 (MQ=255) ttATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACTTCGcc < 1:352707/66‑1 (MQ=255) ttATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGcc < 1:1637602/66‑1 (MQ=255) gCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa < 1:1687416/71‑1 (MQ=255) gCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGAt < 1:497377/39‑1 (MQ=255) cACTTTTCGCACTGCAGACTTATGGAGATTTTCCGGATTCTCGCaa < 1:721361/46‑1 (MQ=255) aCTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa > 1:1911067/1‑69 (MQ=255) ttttCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTg < 1:924775/63‑1 (MQ=255) ttCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaaaa > 1:2504259/1‑43 (MQ=255) ttCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAgcgc > 1:2592771/1‑55 (MQ=255) ttCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAgcgc > 1:1233178/1‑55 (MQ=255) tCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCaaaa > 1:2656384/1‑42 (MQ=255) tCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCaaaa > 1:2453731/1‑42 (MQ=255) tCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCaaaa > 1:107830/1‑42 (MQ=255) cTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTg < 1:755225/55‑1 (MQ=255) gCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACAAAGCCCCACAgcgc > 1:1564438/1‑71 (MQ=255) | CTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACAAAGCCCCACAGCGC > W3110S.gb/2559962‑2560088 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |