Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,560,022 G→A 58.3% P203S (CCT→TCT)  yfeG ← predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,560,0220GA58.3% 6.1 / 27.0 24P203S (CCT→TCT) yfeGpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (5/5);  new base A (5/9);  total (10/14)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

CTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACAAAGCCCCACAGCGC  >  W3110S.gb/2559962‑2560088
                                                            |                                                                  
cttcCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGga                                                            <  1:2201457/69‑1 (MQ=255)
         tGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGa                                                 <  1:908946/71‑1 (MQ=255)
                  tCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCaa                                        <  1:1837754/71‑1 (MQ=255)
                  tCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCaa                                        <  1:1840981/71‑1 (MQ=255)
                       aTTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGt                                   >  1:1245345/1‑71 (MQ=255)
                        ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCa                                         <  1:1081747/64‑1 (MQ=255)
                        ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTg                                                                 <  1:2346482/40‑1 (MQ=255)
                        ttAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGACTg                                                                 <  1:625695/40‑1 (MQ=255)
                          aGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGAtt                                                        >  1:654159/1‑47 (MQ=255)
                                ttATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACTTCGcc                               <  1:352707/66‑1 (MQ=255)
                                ttATCCCCCAGCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGcc                               <  1:1637602/66‑1 (MQ=255)
                                          gCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa                <  1:1687416/71‑1 (MQ=255)
                                          gCACTTTTCGCACTGCAGACTGATGGAGATTTTCCGGAt                                                <  1:497377/39‑1 (MQ=255)
                                           cACTTTTCGCACTGCAGACTTATGGAGATTTTCCGGATTCTCGCaa                                        <  1:721361/46‑1 (MQ=255)
                                            aCTTTTCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACa                >  1:1911067/1‑69 (MQ=255)
                                              ttttCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTg                    <  1:924775/63‑1 (MQ=255)
                                                ttCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCaaaa                                      >  1:2504259/1‑43 (MQ=255)
                                                ttCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAgcgc                          >  1:2592771/1‑55 (MQ=255)
                                                ttCGCACTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAgcgc                          >  1:1233178/1‑55 (MQ=255)
                                                 tCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCaaaa                                      >  1:2656384/1‑42 (MQ=255)
                                                 tCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCaaaa                                      >  1:2453731/1‑42 (MQ=255)
                                                 tCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCaaaa                                      >  1:107830/1‑42 (MQ=255)
                                                      cTGCAGACTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTg                    <  1:755225/55‑1 (MQ=255)
                                                        gCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACAAAGCCCCACAgcgc  >  1:1564438/1‑71 (MQ=255)
                                                            |                                                                  
CTTCCAGCATGGCCCCCATCGCCATTAGCAAATTATCCCCCAGCACTTTTCGCACTGCAGGCTGATGGAGATTTTCCGGATTCTCGCAAAACGTCGCCAGCGCCTGTTGGACAAAGCCCCACAGCGC  >  W3110S.gb/2559962‑2560088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: