| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 505,179 | C→T | 100% | L348L (CTG→TTG) | ushA → | bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 505,179 | 0 | C | T | 77.8% | 49.3 / 11.7 | 27 | L348L (CTG→TTG) | ushA | bifunctional UDP‑sugar hydrolase and 5'‑nucleotidase |
| Reads supporting (aligned to +/- strand): ref base C (0/6); new base T (8/13); total (8/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GAAGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGG > W3110S.gb/505113‑505243 | gAAGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGt < 1:2204998/70‑1 (MQ=255) cGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCACCAAATGATCTCGTTGTTATCAc < 1:1767810/71‑1 (MQ=255) cgTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCAc < 1:1538559/56‑1 (MQ=255) tGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGc < 1:2026867/71‑1 (MQ=255) cTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCa > 1:2603192/1‑70 (MQ=255) cTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCa > 1:2727539/1‑70 (MQ=255) tACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAg < 1:263454/70‑1 (MQ=255) cTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGaa < 1:1132095/55‑1 (MQ=255) tCCTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGc > 1:552548/1‑71 (MQ=255) tCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACaa < 1:508422/57‑1 (MQ=255) cTGAAATCGCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTg > 1:181399/1‑71 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:1574540/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:2275332/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:2303267/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:2676280/71‑1 (MQ=255) gCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:213314/71‑1 (MQ=255) cTGAAAACCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaaa < 1:230227/71‑1 (MQ=255) aaaaCCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATa < 1:1598660/71‑1 (MQ=255) aaaaCCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaaaa < 1:1446504/69‑1 (MQ=255) aCCAGCAAATGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGc > 1:929544/1‑71 (MQ=255) gCAAATGATCTCGCTGTTATCACCGTTCCAGAACaaa < 1:2382537/37‑1 (MQ=255) aaaTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAAcc < 1:1553395/71‑1 (MQ=255) aTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:2682698/56‑1 (MQ=255) aTGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaa < 1:318951/56‑1 (MQ=255) tGATCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGaaaa > 1:2635358/1‑57 (MQ=255) aTCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATgg > 1:2435661/1‑71 (MQ=255) aTCTCGTTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATgg > 1:1922110/1‑71 (MQ=255) | GAAGACGGGAAAAGCGAGCGCGTGCTTTACACTCCTGAAATCGCTGAAAACCAGCAAATGATCTCGCTGTTATCACCGTTCCAGAACAAAGGCAAAGCGCAGCTGGAAGTGAAAATAGGCGAAACCAATGG > W3110S.gb/505113‑505243 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |