| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,611,108 | T→C | 8.3% | F185L (TTC→CTC) | hyfR → | DNA‑binding transcriptional activator, formate sensing |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,611,108 | 0 | T | C | 8.3% | 190.8 / 9.3 | 72 | F185L (TTC→CTC) | hyfR | DNA‑binding transcriptional activator, formate sensing |
| Reads supporting (aligned to +/- strand): ref base T (30/36); new base C (4/2); total (34/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
CAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGC > W3110S.gb/2611043‑2611176 | cAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCgg > 1:1646862/1‑70 (MQ=255)cAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTCACGTCGCTCGTGAGATCCATCATTTTTTCgg < 1:2495139/70‑1 (MQ=255) gTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCt > 1:839802/1‑71 (MQ=255) tGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCg < 1:1814060/66‑1 (MQ=255) tGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCg < 1:2164898/66‑1 (MQ=255) tGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTg > 1:2094545/1‑71 (MQ=255) gCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTg < 1:1671756/70‑1 (MQ=255) cTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGc > 1:2394375/1‑71 (MQ=255) tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCg < 1:2804746/61‑1 (MQ=255) tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCg < 1:2208569/61‑1 (MQ=255) tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCg < 1:75659/61‑1 (MQ=255) tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCt < 1:543874/69‑1 (MQ=255) tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCt < 1:1603869/69‑1 (MQ=255) acaTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCgg < 1:2272394/60‑1 (MQ=255) acaTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTc > 1:701466/1‑62 (MQ=255) acaTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTc > 1:2318933/1‑62 (MQ=255) acaTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcag > 1:1424102/1‑71 (MQ=255) caTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagt < 1:2047912/71‑1 (MQ=255) aTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtc < 1:305431/71‑1 (MQ=255) tCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagt > 1:1311602/1‑69 (MQ=255) tCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagt > 1:1759160/1‑69 (MQ=255) cTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTc > 1:1109522/1‑58 (MQ=255) ttGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTc > 1:2775985/1‑57 (MQ=255) ttGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTc > 1:1822769/1‑57 (MQ=255) ttGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtcag > 1:1939169/1‑70 (MQ=255) ttGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtcag > 1:1353851/1‑70 (MQ=255) ttGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtcag > 1:1311705/1‑70 (MQ=255) ttGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtcagtcag > 1:2799543/1‑70 (MQ=255) gATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtcag < 1:2402746/68‑1 (MQ=255) gATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtcag < 1:1634909/68‑1 (MQ=255) aTCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtcag < 1:1767023/67‑1 (MQ=255) aTCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtcag < 1:280328/67‑1 (MQ=255) aTCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTAt < 1:1595709/70‑1 (MQ=255) aTCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTAt < 1:2014153/70‑1 (MQ=255) aTCTCGACGATCTCATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtcag < 1:2187305/67‑1 (MQ=255) tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCtt < 1:1050297/59‑1 (MQ=255) tctcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa > 1:2741355/1‑68 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATgg < 1:1667081/67‑1 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATgg < 1:637723/67‑1 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGt < 1:95976/68‑1 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGt < 1:1526901/68‑1 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTa > 1:1191023/1‑69 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATg < 1:1501729/66‑1 (MQ=255) gacgaTCTGATCGCTGACGTAGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATg < 1:1270098/66‑1 (MQ=255) gacgaTCTGATCGCTGAAGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTa > 1:2197939/1‑69 (MQ=255) acgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCt < 1:1930365/49‑1 (MQ=255) acgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCt < 1:46518/49‑1 (MQ=255) acgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCt < 1:2401533/49‑1 (MQ=255) acgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCt < 1:2587194/49‑1 (MQ=255) gaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTa > 1:2251268/1‑61 (MQ=255) cTGATCGCTGACGTCGCTCGTGAGATCCATCAttttt > 1:2341036/1‑37 (MQ=255) cTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtcag > 1:1320597/1‑56 (MQ=255) cTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTggg > 1:1711046/1‑68 (MQ=255) cTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGa > 1:569394/1‑71 (MQ=255) cTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGa > 1:769892/1‑71 (MQ=255) aTCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGatc < 1:2357824/70‑1 (MQ=255) aCGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAg < 1:1947936/71‑1 (MQ=255) gTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtca > 1:864988/1‑39 (MQ=255) gCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTa > 1:521032/1‑48 (MQ=255) cTCGTGAGATCCATCATTTTTTCGGTCTGGCTTTAGTCAGTATGGTACTGGGCGATCATCGAAAGAACgag < 1:1320548/71‑1 (MQ=255) cTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACgag < 1:777825/71‑1 (MQ=255) cTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACgag < 1:589604/71‑1 (MQ=255) tGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGt < 1:1795966/42‑1 (MQ=255) tGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGt < 1:1770873/71‑1 (MQ=255) agaTCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGaaa > 1:2433391/1‑58 (MQ=255) agaTCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGtt > 1:2757198/1‑70 (MQ=255) agaTCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGtt > 1:496327/1‑70 (MQ=255) ccATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGcc > 1:1981257/1‑71 (MQ=255) tcatTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCtgt > 1:1826388/1‑71 (MQ=255) tcatTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCtgt < 1:2000676/71‑1 (MQ=255) atTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa > 1:2231152/1‑52 (MQ=255) atTTTTTCGGTCTGGCTTCAGTAAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTgtgg > 1:1490114/1‑71 (MQ=255) atTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGatca > 1:2434365/1‑43 (MQ=255) tttCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGc < 1:2061900/70‑1 (MQ=255) | CAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTGC > W3110S.gb/2611043‑2611176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |