| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,632,142 | C→T | 22.7% | M195I (ATG→ATA) | guaB ← | IMP dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,632,142 | 0 | C | T | 22.7% | 41.0 / 8.6 | 22 | M195I (ATG→ATA) | guaB | IMP dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (9/8); new base T (3/2); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGC > W3110S.gb/2632073‑2632210 | cAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCa < 1:1802164/71‑1 (MQ=255) gCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGa < 1:2506810/71‑1 (MQ=255) tGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGcc > 1:853310/1‑65 (MQ=255) tGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGcc > 1:1387930/1‑65 (MQ=255) gCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATTATGCCGATCAgg > 1:511074/1‑71 (MQ=255) cAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGt < 1:1090804/70‑1 (MQ=255) cAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGt < 1:1089657/70‑1 (MQ=255) ttCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAac < 1:93993/71‑1 (MQ=255) cGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATTATGCCGATCAGGTGGAATTCGTCATCAacca > 1:1680575/1‑71 (MQ=255) cGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAacc > 1:365783/1‑70 (MQ=255) ggTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATTATGCCGAt < 1:2543565/47‑1 (MQ=255) ttACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAg > 1:1972240/1‑71 (MQ=255) cGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAgcgc < 1:2223863/71‑1 (MQ=255) cGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAgcgc > 1:1732669/1‑71 (MQ=255) gTTCCGCTTTCTGGAAGTCTTTCACGGTGATTATGCCGATCAGGTGGAATTCGTCATCAac < 1:1173488/61‑1 (MQ=255) cTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAac < 1:2588453/55‑1 (MQ=255) ttCACGGTGATTATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTg > 1:1245141/1‑71 (MQ=255) tCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCaa > 1:409363/1‑39 (MQ=255) tCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCaa > 1:999514/1‑39 (MQ=255) aTCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCtt > 1:1695077/1‑43 (MQ=255) aTCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGttttt < 1:1705017/58‑1 (MQ=255) aTCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGc > 1:2801415/1‑71 (MQ=255) | CAGACGGCCTTGCTCGTCTTTACAGGCGTTCGGTTTACGTTCCGCTTTCTGGAAGTCTTTCACGGTGATCATGCCGATCAGGTGGAATTCGTCATCAACCACCAGCGCTTTTTCAACGCGTTTTTCGTGCATTTTTGC > W3110S.gb/2632073‑2632210 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |