| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,646,699 | C→G | 73.9% | Q1415H (CAG→CAC) | yfhM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,646,699 | 0 | C | G | 73.9% | 34.4 / 9.6 | 23 | Q1415H (CAG→CAC) | yfhM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (2/4); new base G (3/14); total (5/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAG > W3110S.gb/2646635‑2646765 | tACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGaa < 1:332956/71‑1 (MQ=255) gggCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGaa < 1:2529657/68‑1 (MQ=255) gAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAACCGCctgc < 1:1361885/52‑1 (MQ=255) gAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAgt < 1:2538108/61‑1 (MQ=255) gAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGtt < 1:2786275/66‑1 (MQ=255) aacGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAgtgt > 1:770985/1‑59 (MQ=255) acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTTGTGCGAAAGTGTTTTCAGCAAAgtg < 1:1689657/71‑1 (MQ=255) acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:964543/71‑1 (MQ=255) acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:315467/71‑1 (MQ=255) acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:2756988/71‑1 (MQ=255) acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAgtg < 1:1303888/71‑1 (MQ=255) acGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAgtg < 1:1407411/71‑1 (MQ=255) gTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:2178185/67‑1 (MQ=255) ttACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAgtgt < 1:2592906/67‑1 (MQ=255) tCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTa > 1:615850/1‑62 (MQ=255) gATAGCCAGCGTTCACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAg < 1:311946/71‑1 (MQ=255) cAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCaaa > 1:1850940/1‑42 (MQ=255) cACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCggg > 1:114043/1‑52 (MQ=255) cACCAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTttcttc > 1:2625195/1‑70 (MQ=255) ccAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAgtg < 1:1533600/36‑1 (MQ=255) cAAACGCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGc < 1:2752985/71‑1 (MQ=255) aCGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAg < 1:252886/71‑1 (MQ=255) gCCTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGga < 1:739552/71‑1 (MQ=255) ccTGGTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGgag < 1:948365/71‑1 (MQ=255) | TACGGGCAGCCAGGAACAACGCGTTACTTTCCTGCGTCGATAGCCAGCGTTCACCAAACGCCTGCTGCGAAAGTGTGTTCAGCAAAGTGTACTGCTCATCGGGTAGCAGTTTATTTTCTTCCAGCAAGGAG > W3110S.gb/2646635‑2646765 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |