| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,732,756 | T→A | 65.8% | D25V (GAC→GTC) | clpB ← | protein disaggregation chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,732,756 | 0 | T | A | 65.8% | 37.4 / 24.9 | 32 | D25V (GAC→GTC) | clpB | protein disaggregation chaperone |
| Reads supporting (aligned to +/- strand): ref base T (3/8); new base A (9/12); total (12/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGAACCCCCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGTCGTGCCCGAGTGCAAGTGATTGGGCATCGGCAAGAGCAAGCTGGAATTTATTAGTAAGACGATCCA > W3110S.gb/2732694‑2732822 | ccGAACCCCCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCg < 1:2103211/71‑1 (MQ=255) cccccTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTg > 1:966686/1‑70 (MQ=255) ttCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGTTAAATTGGTTGTCGTGCCCg < 1:361319/61‑1 (MQ=255) tCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCa > 1:1880506/1‑71 (MQ=255) agGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGTCGTGCCCGAGTGCAAGTGATTGGGCATCGGc < 1:614228/71‑1 (MQ=255) agGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGTCGTGCCCGAGTGCAAGTGATTGGGCATCGGc < 1:2294418/71‑1 (MQ=255) agGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGTCGTGCCCGAGTGCAAGTGATTGGGCATCGGc < 1:2046053/71‑1 (MQ=255) agGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCGGc < 1:1037076/71‑1 (MQ=255) gggCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCGGc < 1:2222110/70‑1 (MQ=255) gcgcTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATAGGc > 1:67243/1‑68 (MQ=255) gcTCATTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCGGCAagag > 1:2146758/1‑71 (MQ=255) aTTAAATGAAGTGGTTCGATAAATTGGTTGTCGTGCCCGAGTGCAAGTGATTGGGCa < 1:98341/57‑1 (MQ=255) aTTAAATGAAGTGGTTCGATAAATTGGTTGTCGTGCCCGAGTGCAAGTGATTGGGCa < 1:101631/57‑1 (MQ=255) aTTAAATGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCGGCAAGATCaa < 1:1736817/70‑1 (MQ=255) tAAATGAAGTGGTTCGATAAATTGGTTGTCGTGCCCGAGTGCAAGTGATTGGGCATCGGCAagag > 1:1324725/1‑65 (MQ=255) aaaTGAAGTGGTTCGATAAATTGGTTGTCGTGCCCGAGTGCa > 1:672559/1‑42 (MQ=255) aaaTGAAGTGGTTCGATAAATTGGTTGTCGTGCCCGAGTGCAAGTGATTGGGCATCGGCAAGAGCAAGCTg > 1:2069720/1‑71 (MQ=255) aaaTGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCGGCAAGAGCAAGCTg > 1:1151077/1‑71 (MQ=255) aaTGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGt < 1:1257645/44‑1 (MQ=255) aaTGAAGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCGGCAAGAGCAAGCTgg < 1:2020668/71‑1 (MQ=255) aGTGGTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCGGCAAGAGCAAGCTGGAAtt > 1:56140/1‑70 (MQ=255) ggTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCGGCAATAGCAAGCTGGaa > 1:484759/1‑65 (MQ=255) gTTCGATAAATTGGTTGTCGTGCCCGAGTGCAAGt < 1:518265/35‑1 (MQ=255) gTTCGATAAATTGGTTGTCGTGCCCGAGTGCAAGt < 1:385068/35‑1 (MQ=255) gTTCGATAAATTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCGGCAAGAGCAAGCTGGAATttat > 1:108740/1‑69 (MQ=255) gATAAATTGGGTGACGTGCCCGAGTGCAAGTGATTGGGCATCGGc < 1:1225021/45‑1 (MQ=255) aaTTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCGGCAAGAGCAAGCTGGAATTTATTAGTAAGAc > 1:858163/1‑70 (MQ=255) aaTTGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCGGCAAGAGCAAGCTGGAATTTATTAGTAAGACg < 1:1589650/71‑1 (MQ=255) ttGGTTGACGTGCCCGAGTGCAAGTGATTGGGCATCGGCAAGAGCAAGCTGGAATttat < 1:2119161/59‑1 (MQ=255) ggTTGACGTGCCCGAGTGCAAGTGATTGGGCATCGGCAAGAGCAAGCTGGAATTTATTAGTAAGACGATc < 1:39603/70‑1 (MQ=255) gTTGACGTGCCCGAGTGCAAGTGATTGGGCATCGGCAAGAGCAAGCTGGAATTTATTAGTAAGACGATcc < 1:737174/70‑1 (MQ=255) gTTGACGTGCCCGAGTGCAAGTGATTGGGCATCGGCAAGAGCAAGCTGGAATTTATTAGTAAGACGATCCa < 1:481560/71‑1 (MQ=255) | CCGAACCCCCTTCCTGATTCAGCAGGGCGCTCATTAAATGAAGTGGTTCGATAAATTGGTTGTCGTGCCCGAGTGCAAGTGATTGGGCATCGGCAAGAGCAAGCTGGAATTTATTAGTAAGACGATCCA > W3110S.gb/2732694‑2732822 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |