Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,770,406 T→C 35.3% intergenic (+135/+90) yfjT → / ← yfjU hypothetical protein/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,770,4060TC35.3% 11.2 / 13.5 17intergenic (+135/+90)yfjT/yfjUhypothetical protein/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (4/7);  new base C (4/2);  total (8/9)
Fisher's exact test for biased strand distribution p-value = 3.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TAATCTCCAGAGGTAGGCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGA  >  W3110S.gb/2770339‑2770462
                                                                   |                                                        
tAATCTCCAGAGGTAGGCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTa                                                        >  1:2667270/1‑70 (MQ=255)
            gTAGGCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATCTATCTTTTTATGGCt                                           >  1:917213/1‑71 (MQ=255)
               ggCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATCTATCTTTTTTTGGCTGt                                         >  1:72338/1‑70 (MQ=255)
                     gTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTcc                                  <  1:2066282/71‑1 (MQ=255)
                      tACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCg                                 >  1:2161502/1‑71 (MQ=255)
                                      gcgcAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCtg                 <  1:1877971/71‑1 (MQ=255)
                                      gcgcAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCtg                 <  1:1352322/71‑1 (MQ=255)
                                            tGGTCAGTTTTTTTCAGATATATCTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTaa           <  1:192067/71‑1 (MQ=255)
                                            tGGTCAGTTTTTTTCAGATATATCTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTaa           <  1:1641798/71‑1 (MQ=255)
                                              gTCAGTTTTTTTCAGATATATCTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTaaaa         >  1:776493/1‑71 (MQ=255)
                                               tCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAgcg                        <  1:2789508/55‑1 (MQ=255)
                                               tCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAAc        <  1:225173/71‑1 (MQ=255)
                                                    ttttttCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCa                      >  1:1044078/1‑52 (MQ=255)
                                                    ttttttCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCtg                 <  1:1990635/57‑1 (MQ=255)
                                                     tttttCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTaaaa         >  1:1510145/1‑64 (MQ=255)
                                                     tttttCAGATATATCTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGa  >  1:778703/1‑71 (MQ=255)
                                                                tatTTATCTTTTTTTGGCTGTAAGCTCCTCTGTGAGCGCAGGCTGTGTTAAAACTCCTg   <  1:47179/59‑1 (MQ=255)
                                                                   |                                                        
TAATCTCCAGAGGTAGGCCACGTACTTTCGAACGATGAGCGCAGTGGTCAGTTTTTTTCAGATATATTTATCTTTTTTTGGCTGTAAGCTCCGCTGTGAGCGCAGGCTGTGTTAAAACTCCTGA  >  W3110S.gb/2770339‑2770462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑