| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,841,701 | C→T | 48.8% | L134L (CTG→CTA) | hycH ← | protein required for maturation of hydrogenase 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,841,701 | 0 | C | T | 48.8% | 6.6 / 120.9 | 82 | L134L (CTG→CTA) | hycH | protein required for maturation of hydrogenase 3 |
| Reads supporting (aligned to +/- strand): ref base C (17/25); new base T (21/19); total (38/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01 | |||||||||||
GCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTA > W3110S.gb/2841632‑2841770 | gCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCa < 1:1842003/71‑1 (MQ=255) agcagcGGACCTGCGCCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGtc > 1:2179136/1‑71 (MQ=255) gcgcCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATc > 1:534589/1‑70 (MQ=255) cgcCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCa < 1:1055145/70‑1 (MQ=255) cgcCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCa < 1:1323873/70‑1 (MQ=255) gcCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCaaa < 1:1162027/71‑1 (MQ=255) gcCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCaaa < 1:597857/71‑1 (MQ=255) gcCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCaaa < 1:932542/71‑1 (MQ=255) catcatCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATa < 1:1178582/71‑1 (MQ=255) catcatCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATa < 1:960766/71‑1 (MQ=255) catcGCCCATCATGCTATTGCCTACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCaaa < 1:1611963/66‑1 (MQ=255) catcGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGa > 1:420109/1‑70 (MQ=255) atcGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCAc > 1:559600/1‑58 (MQ=255) atcGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATg > 1:786917/1‑71 (MQ=255) atcGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATg > 1:2347433/1‑71 (MQ=255) atcGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATg > 1:2725606/1‑71 (MQ=255) catcatGCTATTGCTAACACAGAGTAAAACGTCAGTCACGTAGTctcc < 1:1004390/48‑1 (MQ=255) catcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTctcc < 1:33338/48‑1 (MQ=255) catcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTctcc < 1:1954444/48‑1 (MQ=255) catcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGttt > 1:77745/1‑71 (MQ=255) catcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGttt < 1:1879755/71‑1 (MQ=255) atcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGtttt < 1:1136048/71‑1 (MQ=255) atcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGtct < 1:1975283/45‑1 (MQ=255) atcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGtct < 1:2231660/45‑1 (MQ=255) atcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGtct < 1:1182351/45‑1 (MQ=255) atcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGtct < 1:1971938/45‑1 (MQ=255) tcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCAcc < 1:2515437/51‑1 (MQ=255) tcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCa < 1:1677653/55‑1 (MQ=255) tcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCa < 1:773884/55‑1 (MQ=255) tcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAg < 1:1456910/60‑1 (MQ=255) tcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGAt < 1:1348966/62‑1 (MQ=255) tcatGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGAt < 1:399207/62‑1 (MQ=255) catGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTcc > 1:1569809/1‑71 (MQ=255) atGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCaaa > 1:799888/1‑55 (MQ=255) atGCTATTGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCaaa > 1:1306666/1‑55 (MQ=255) atGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCa < 1:1323067/53‑1 (MQ=255) atGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCt < 1:1825333/71‑1 (MQ=255) aTTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatg > 1:131927/1‑70 (MQ=255) ttGCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatg < 1:1728267/69‑1 (MQ=255) ttGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCaa < 1:150087/48‑1 (MQ=255) ttGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCaa < 1:2470685/48‑1 (MQ=255) ttGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCaa < 1:789448/48‑1 (MQ=255) ttGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGaa > 1:746427/1‑71 (MQ=255) gCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtga > 1:2150415/1‑65 (MQ=255) gCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtga > 1:493387/1‑65 (MQ=255) gCCAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAat > 1:1224539/1‑70 (MQ=255) cAACACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATc < 1:51135/71‑1 (MQ=255) cAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATc < 1:1402991/71‑1 (MQ=255) aaCACAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCa > 1:94796/1‑71 (MQ=255) aaCACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGa > 1:761087/1‑48 (MQ=255) acacAGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCAt > 1:639098/1‑71 (MQ=255) cacaGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCtgatg < 1:1197515/62‑1 (MQ=255) acaGAGTAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATg > 1:1452976/1‑70 (MQ=255) agTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATgcagca < 1:1426339/71‑1 (MQ=255) gTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATgcagca < 1:2536687/70‑1 (MQ=255) tAAAACGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAatat < 1:2251000/60‑1 (MQ=255) tAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATa < 1:1780749/37‑1 (MQ=255) tAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATa < 1:2252924/71‑1 (MQ=255) aaaCGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACt > 1:487079/1‑71 (MQ=255) aaaCGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACt > 1:2585710/1‑71 (MQ=255) aaaCGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACt > 1:697040/1‑71 (MQ=255) aaaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACt > 1:504042/1‑71 (MQ=255) aaCGTCAGTCACGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACtt < 1:777695/71‑1 (MQ=255) aaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACtt < 1:1006111/71‑1 (MQ=255) aaCGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACtt < 1:2056601/71‑1 (MQ=255) cagtcaCGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAg > 1:2047526/1‑71 (MQ=255) cagtcaCGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAg > 1:1771832/1‑71 (MQ=255) cagtcaCGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAg > 1:2050789/1‑71 (MQ=255) cagtcaCGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACa > 1:166216/1‑70 (MQ=255) caCGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGaa > 1:1832536/1‑45 (MQ=255) caCGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGaa > 1:2576933/1‑45 (MQ=255) caCGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCAtt > 1:2081361/1‑71 (MQ=255) caCGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCAtt > 1:201117/1‑71 (MQ=255) aCGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAata > 1:2504804/1‑46 (MQ=255) aCGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAata > 1:1288972/1‑46 (MQ=255) aCGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTg < 1:2047387/71‑1 (MQ=255) aCGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTg > 1:201444/1‑71 (MQ=255) aCGTAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTg < 1:1285180/71‑1 (MQ=255) aCGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCa > 1:2513689/1‑68 (MQ=255) aCGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTg < 1:767812/71‑1 (MQ=255) gCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGaa > 1:2186068/1‑42 (MQ=255) gCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTa > 1:972602/1‑71 (MQ=255) | GCCAGCAGCGGACCTGCGCCATCATCGCCCATCATGCTATTGCCAACACAGAGTAAAACGTCAGTCACGCAGTCTCCTCACCATCAAATAGATGGCGTTTTCCTGATGAATATCATGCAGCATACTTAACAGCATTGTA > W3110S.gb/2841632‑2841770 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |