| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 540,085 | C→T | 12.9% | I99I (ATC→ATT) | ybbY → | predicted uracil/xanthine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 540,085 | 0 | C | T | 12.9% | 82.8 / 3.7 | 31 | I99I (ATC→ATT) | ybbY | predicted uracil/xanthine transporter |
| Reads supporting (aligned to +/- strand): ref base C (17/10); new base T (2/2); total (19/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGTGGTGGGGAACCATCCTTACTATCACCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATCGCCACCAGCCTGGCAGTGGGGATTGCACTCTCCGGCGTGCTGACGATGTTGATTGGTTTTAGCGGAT > W3110S.gb/540018‑540152 | tgtgGTGGGGAACCATCCTTACTATCACCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATCGc > 1:1239127/1‑70 (MQ=255) ggggAACCATCCTTACTATCACCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATCGCCACCAGc > 1:1998217/1‑71 (MQ=255) gAACCATCCTTACTATCACCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATCGCCACCAGCCTg > 1:195118/1‑71 (MQ=255) aCCATCCTTACTATCACCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATCGCCACCAGCCTGGc < 1:1472112/71‑1 (MQ=255) aCCATCCTTACTATCACCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATCGCCACCAGCCTGGc < 1:2409530/71‑1 (MQ=255) ccATCCTTACTATCACCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATTGCCACCAGCCTgg > 1:1106417/1‑69 (MQ=255) ccATCCTTACTATCACCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATTGCCACCAGCCTgg > 1:2020454/1‑69 (MQ=255) aTCCTTACTATCACCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATCGCCACCAg < 1:2436332/62‑1 (MQ=255) cTTACTATCACCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATCGCCACCAGCCTGGCAGTggg < 1:1952531/71‑1 (MQ=255) cTTACTATCACCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATCGCCACCAGCCTGGCAGTgg > 1:2395794/1‑70 (MQ=255) aTCACCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATCGCCACCAGCCTGGCAGt > 1:2787260/1‑62 (MQ=255) tCACCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATCGcca > 1:863037/1‑48 (MQ=255) aCCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATCGcca > 1:658999/1‑46 (MQ=255) aCCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATCGCCACCAGCCTGGCAg > 1:2026256/1‑58 (MQ=255) cccTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATCGCCACCAGCCTgg < 1:1419471/54‑1 (MQ=255) ggTGAAGCATCGCGCGGGACACCGATCAACGATATCGCCCCCAGcc > 1:732145/1‑46 (MQ=255) ggTGAAGCATCGCGCGGGACACCGATCAACGATATCGCCACCAGcc > 1:2005237/1‑46 (MQ=255) ggTGAAGCATCGCGCGGGACACCGATCAACGATATCGCCACCAGcc > 1:1527256/1‑46 (MQ=255) ggTGAAGCATCGCGCGGGACACCGATCAACGATATCGCCACCAGCCTGGCAGTGGGGa > 1:2544300/1‑58 (MQ=255) gCATCGCGCGGGACACCGATCAACGATATCGCCACCAGCCTGGCAg > 1:2298850/1‑46 (MQ=255) tCGCGCGGGACACCGATCAACGATATCGCCACCAGCCTGGc < 1:1954688/41‑1 (MQ=255) tCGCGCGGGACACCGATCAACGATATCGCCACCAGCCTGGCAGTGGGGATTg < 1:1710905/52‑1 (MQ=255) tCGCGCGGGACACCGATCAACGATATCGCCACCAGCCTGGCAGTGGGGATTGCACTCTCCGGCGTGCTGAc < 1:2174586/71‑1 (MQ=255) tCGCGCGGGACACCGATCAACGATATCGCCACCAGCCTGGCAGTGGGGATTGCACTCTCCGGCCTGCTGAc < 1:298884/71‑1 (MQ=255) acacCGATCAACGATATCGCCACCAGCCTGGCAGTGGGGATTGCACTCTCCGGCGTGCTGACGATGTTGAt > 1:190879/1‑71 (MQ=255) acCGATCAACGATATCGCCACCAGCCTGGCAGTGGGGATTGCACTCTCCGGCGTGCTGACGATGTTGATTg > 1:2757123/1‑71 (MQ=255) cGATCAACGATATCGCCACCAGCCTGGCAGTGGGGATTGCACTCTCCGGCGTGCTGACGATGTTGATTGGt > 1:2326038/1‑71 (MQ=255) cGATATTGCCACCAGCCTGGCAGTGGGGATTGCACTCTCCGGCGTGCTGACGATGTTGATTGGTTTTAGCg < 1:2428/71‑1 (MQ=255) tatTGCCACCAGCCTGGCAGTGGGGATTGCACTCTCCGGCGTGCTGACGATGTTGATTGGTTTTAGCGGAt < 1:1293949/71‑1 (MQ=255) tatCGCCACCAGCCTGGCAGTGGGGATTGCACTCTCCGGCGTGCTGACGATGTTGATTGGTTTTAGCGGAt < 1:1057857/71‑1 (MQ=255) atCGCCACCAGCCTGGCAGTGGGGATTGCACTCTCCGGCGTGCTGACGATGTTGATTGGTTTTAGCGGAt > 1:2147724/1‑70 (MQ=255) | TGTGGTGGGGAACCATCCTTACTATCACCCTTGGTGAAGCATCGCGCGGGACACCGATCAACGATATCGCCACCAGCCTGGCAGTGGGGATTGCACTCTCCGGCGTGCTGACGATGTTGATTGGTTTTAGCGGAT > W3110S.gb/540018‑540152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |