| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,863,108 | T→C | 19.4% | L73L (TTA→CTA) | ygbM → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,863,108 | 0 | T | C | 19.4% | 61.8 / 11.5 | 31 | L73L (TTA→CTA) | ygbM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (10/15); new base C (3/3); total (13/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
CTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGC‑GGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCAT > W3110S.gb/2863057‑2863174 | cTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGC‑GGGGGAATGGGGACTATCCGCCCTTCCCGGAc > 1:1736280/1‑70 (MQ=255) ggCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGGAATGGGGATTATCCGCCCTTCCCGGACGt > 1:664935/1‑71 (MQ=255) ttCAATACCGCTCCCGGAGATATTAATGC‑GGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGa < 1:1295417/71‑1 (MQ=255) ttCAATACCGCTCCCGGAGATATTAATGC‑GGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGa < 1:2454021/71‑1 (MQ=255) cAATACCGCTCCCGGAGATATTAATGC‑GGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGaa > 1:1275266/1‑70 (MQ=255) aaTACCGCTCCCGGAGATATTAATGC‑GGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAg < 1:474259/70‑1 (MQ=255) aTACCGCTCCCGGAGATATTAATGC‑GGGTGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGca < 1:1371407/71‑1 (MQ=255) aTACCGCTCCCGGAGATATTAATGC‑GGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGca < 1:1708873/71‑1 (MQ=255) aTACCGCTCCCGGAGATATTAATGC‑GGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGca < 1:2372522/71‑1 (MQ=255) aCCGCTCCCGGAGATATTAATGC‑GGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGcaca > 1:991009/1‑71 (MQ=255) aCCGCTCCCGGAGATATTAATGC‑GGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAGcaca > 1:2502862/1‑71 (MQ=255) cTCCCGGAGATATTAATGC‑GGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCa < 1:2380494/71‑1 (MQ=255) agaTATTAATGC‑GGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCg < 1:1935604/71‑1 (MQ=255) gaTATTAATGC‑GGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGa < 1:496105/71‑1 (MQ=255) atTAATGC‑GGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGa > 1:1833363/1‑62 (MQ=255) ttAATGC‑GGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCa > 1:1259542/1‑46 (MQ=255) ttAATGC‑GGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACg < 1:923429/57‑1 (MQ=255) ttAATGC‑GGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGAc < 1:1489219/62‑1 (MQ=255) c‑GGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTg < 1:1318606/71‑1 (MQ=255) c‑GGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAGcaca > 1:2483491/1‑49 (MQ=255) gggAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa < 1:466800/71‑1 (MQ=255) gggAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa < 1:1625130/71‑1 (MQ=255) gggAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa < 1:2599910/71‑1 (MQ=255) tGGGGATTATCCGCCCTTCCCGGACGTGAGCACGa < 1:828836/35‑1 (MQ=255) tGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAg < 1:1423859/37‑1 (MQ=255) tGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCa < 1:1750495/45‑1 (MQ=255) ggggATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa > 1:1774809/1‑65 (MQ=255) ggggATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa > 1:2549630/1‑65 (MQ=255) gggATTATCCGCCCTTCCCGGACGTGAGCACGAAGcaca > 1:1810540/1‑39 (MQ=255) gggATTATCCGCCCTTCCCGGACGTGAGCACGAAGcaca > 1:1686810/1‑39 (MQ=255) gggATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCAt > 1:1076532/1‑71 (MQ=255) | CTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGC‑GGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCAT > W3110S.gb/2863057‑2863174 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |