| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,875,612 | G→A | 64.1% | D126N (GAT→AAT) | iap → | aminopeptidase in alkaline phosphatase isozyme conversion |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,875,612 | 0 | G | A | 64.1% | 33.4 / 38.4 | 39 | D126N (GAT→AAT) | iap | aminopeptidase in alkaline phosphatase isozyme conversion |
| Reads supporting (aligned to +/- strand): ref base G (10/4); new base A (14/11); total (24/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
ATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGG > W3110S.gb/2875547‑2875676 | aTGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAATGCt < 1:1983182/71‑1 (MQ=255) gAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGa > 1:2329086/1‑65 (MQ=255) aaaGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGa > 1:86363/1‑71 (MQ=255) aaaGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGa > 1:748839/1‑71 (MQ=255) gcgcCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGc > 1:1093075/1‑71 (MQ=255) gcgcCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGc > 1:663274/1‑71 (MQ=255) cgccgcAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGcc < 1:2492148/71‑1 (MQ=255) agATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTcggc > 1:911470/1‑71 (MQ=255) agATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTcggc < 1:2622448/71‑1 (MQ=255) agATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTcggc > 1:2224822/1‑71 (MQ=255) atcatcATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCt < 1:434368/48‑1 (MQ=255) tcatcaTTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCggg > 1:1637913/1‑71 (MQ=255) catcatTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTg > 1:2266001/1‑47 (MQ=255) catcatTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAATGCTGa > 1:2348832/1‑48 (MQ=255) catcatTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGa > 1:869225/1‑54 (MQ=255) catcatTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGc > 1:1142343/1‑71 (MQ=255) catcatTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGc > 1:702868/1‑71 (MQ=255) catcatTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGc > 1:2685941/1‑71 (MQ=255) tcatTATGGCGCATCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCt < 1:2765176/70‑1 (MQ=255) catTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGa > 1:2303889/1‑71 (MQ=255) catTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGa > 1:78161/1‑71 (MQ=255) ttATGGCGCATCTGGATACTTACGCCCCGCTGAGCAATGCt < 1:2419900/41‑1 (MQ=255) tATGGCGCATCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGt > 1:1465360/1‑71 (MQ=255) aTGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGt > 1:302760/1‑70 (MQ=255) gcgcATCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTAc < 1:1151853/70‑1 (MQ=255) gcgcATCTGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTAc > 1:102655/1‑70 (MQ=255) tGGATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGTCAATCTCGGCGGGCTGACGTTACAAGGAATg > 1:122573/1‑71 (MQ=255) gATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATgg < 1:1996213/70‑1 (MQ=255) gATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATgg < 1:413503/70‑1 (MQ=255) gATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATg < 1:1436843/69‑1 (MQ=255) gATACTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATg < 1:821487/69‑1 (MQ=255) cTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAgg < 1:1558599/61‑1 (MQ=255) cTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatgat < 1:593767/71‑1 (MQ=255) cTTACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGgatgat < 1:2397945/71‑1 (MQ=255) tACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAgg < 1:1953803/59‑1 (MQ=255) tACGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATaa > 1:2220413/1‑71 (MQ=255) aCGCCCCGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAc > 1:1183603/1‑71 (MQ=255) cGCTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAAcgccg > 1:964874/1‑69 (MQ=255) cTGAGCAATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAgg > 1:226660/1‑71 (MQ=255) | ATGAAGGCAAAGCGCCGCAGCAGATCATCATTATGGCGCATCTGGATACTTACGCCCCGCTGAGCGATGCTGACGCCGATGCCAATCTCGGCGGGCTGACGTTACAAGGAATGGATGATAACGCCGCAGG > W3110S.gb/2875547‑2875676 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |