Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	2,894,340	C→T	73.7%	W32* (TGG→TGA)	ygcQ ←	predicted flavoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,894,340	0	C	T	73.7%	56.2 / 24.7	38	W32* (TGG→TGA)	ygcQ	predicted flavoprotein
Reads supporting (aligned to +/- strand):  ref base C (8/2);  new base T (16/12);  total (24/14)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01

GCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTC  >  W3110S.gb/2894276‑2894403                                                                 |                                                                gCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTgc                                                           <  1:2669828/71‑1 (MQ=255)  cTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTgcg                                                          <  1:1275395/71‑1 (MQ=255)     ccACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGcc                                                        <  1:1572057/70‑1 (MQ=255)             ccaccaACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGt                                                   >  1:783059/1‑67 (MQ=255)                    cGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGa                                        <  1:851064/71‑1 (MQ=255)                      cATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGaaa                                      >  1:2123543/1‑71 (MQ=255)                        tCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATc                                    <  1:1974045/71‑1 (MQ=255)                          cAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCt                                  >  1:712472/1‑71 (MQ=255)                          cAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCt                                  >  1:2602436/1‑71 (MQ=255)                          cAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCt                                  >  1:2599247/1‑71 (MQ=255)                          cAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAg                                                              >  1:563881/1‑43 (MQ=255)                          cAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACaa                                               >  1:2781829/1‑58 (MQ=255)                          cAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACaa                                               >  1:1768344/1‑58 (MQ=255)                            gCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGc                                           >  1:2672667/1‑60 (MQ=255)                             cACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTgcg                               >  1:395/1‑71 (MQ=255)                             cACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTgcg                               >  1:1363587/1‑71 (MQ=255)                               ccTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCa                                                               >  1:153145/1‑37 (MQ=255)                               ccTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCa                                                               >  1:628936/1‑37 (MQ=255)                               ccTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCa                                                               >  1:696850/1‑37 (MQ=255)                                cTGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGaa                                       <  1:466385/60‑1 (MQ=255)                                 tGTTCCGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGcgccgc                           >  1:2264604/1‑71 (MQ=255)                                     ccGCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCgccagc                       >  1:2296695/1‑71 (MQ=255)                                       gCCACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCgccagcca                     <  1:1647052/71‑1 (MQ=255)                                        ccACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACGACCGCTGAAATCCTGCGCCGccagccag                    <  1:1816354/71‑1 (MQ=255)                                        ccACTACAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGccagccag                    <  1:2775445/71‑1 (MQ=255)                                             aCAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATc                                    >  1:2202147/1‑50 (MQ=255)                                              cAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGc               >  1:1096917/1‑70 (MQ=255)                                              cAGGCTGAGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGc               >  1:1392198/1‑70 (MQ=255)                                              cAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGc               >  1:2597115/1‑70 (MQ=255)                                              cAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCt              <  1:1607316/71‑1 (MQ=255)                                                 gCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTAt            <  1:444861/70‑1 (MQ=255)                                                     aGGTTCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCgg       <  1:1787372/71‑1 (MQ=255)                                                       gTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTa             >  1:803415/1‑63 (MQ=255)                                                        ttCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCt              <  1:246450/61‑1 (MQ=255)                                                         tCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCAtt   >  1:582452/1‑71 (MQ=255)                                                         tCAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCAtt   >  1:777875/1‑71 (MQ=255)                                                          cAATCTGTCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATtc  >  1:1514126/1‑71 (MQ=255)                                                            aTCTGTCAGTGCGCCAGGGTACGACCGCTGAAATCCTGCGCCGccagccag                    <  1:1819651/51‑1 (MQ=39)                                                                 |                                                                GCTGCCACTGTTCCACCAACGCATCCAGCACCTGTTCCGCCACTACAGGCTGAGGTTCAATCTGCCAGTGCGCCAGGGTACAACCGCTGAAATCCTGCGCCGCCAGCCAGCTTGCTATTGCGGCATTC  >  W3110S.gb/2894276‑2894403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑