| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,902,330 | A→T | 15.4% | intergenic (+300/+1073) | ygcE → / ← ygcF | predicted kinase/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,902,330 | 0 | A | T | 15.4% | 59.5 / 5.6 | 26 | intergenic (+300/+1073) | ygcE/ygcF | predicted kinase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (10/12); new base T (2/2); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
CTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACC > W3110S.gb/2902260‑2902398 | cTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGtata < 1:2663279/71‑1 (MQ=255)cTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGtata < 1:2337613/71‑1 (MQ=255)cTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGtata < 1:1934152/71‑1 (MQ=255) tATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATag > 1:2032559/1‑69 (MQ=255) aaTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCgg > 1:1085747/1‑71 (MQ=255) aaTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCgg > 1:2781536/1‑71 (MQ=255) ttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTAt > 1:1192755/1‑71 (MQ=255) cACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATaga > 1:2478458/1‑60 (MQ=255) cACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATg > 1:1087739/1‑70 (MQ=255) cTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATTGAGGCGGTTATGTaa < 1:2367633/71‑1 (MQ=255) cTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTaa < 1:1603657/71‑1 (MQ=255) aaaaGTAAGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATaga > 1:807928/2‑56 (MQ=255) aaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAc < 1:925798/70‑1 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGtata < 1:756111/47‑1 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACt < 1:2250534/69‑1 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTc < 1:2325520/70‑1 (MQ=255) aaCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCAtt < 1:1702775/71‑1 (MQ=255) tGGATATCTTTATTCATAAGAACTATTCATCAACAGCGtata < 1:2644466/42‑1 (MQ=255) ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAACACCACTCATTAGcc > 1:132175/1‑71 (MQ=255) ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGcc > 1:1528577/1‑71 (MQ=255) atCTTTATTCATAAGAACTATTCATCAACAGCGtata > 1:434068/1‑37 (MQ=255) tCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCaa > 1:776418/1‑70 (MQ=255) tttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTaaaa > 1:1333810/1‑51 (MQ=255) tATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTaaa > 1:2048101/1‑48 (MQ=255) cATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGa > 1:2153927/1‑70 (MQ=255) ttCATCAACAGCGTATAGAGGCGGTTATGTAAAACCAGTCATTAGCCTCAAAACAAAGACAAACTTCCAg < 1:2037695/70‑1 (MQ=255) catcaACAGCGTATTGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGAtt > 1:730954/1‑71 (MQ=255) catcaACAGCGTATTGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGAt > 1:1931549/1‑70 (MQ=255) atcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGAttt < 1:1504512/71‑1 (MQ=255) atcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGAttt < 1:63543/71‑1 (MQ=255) atcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGAtt < 1:45557/70‑1 (MQ=255) atcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGAtt < 1:2249370/70‑1 (MQ=255) tcaACAGCGTATTGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACa < 1:2471657/57‑1 (MQ=255) tcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGAttt < 1:2639113/70‑1 (MQ=255) aaCAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATttat < 1:1752726/70‑1 (MQ=255) taGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAAcc < 1:1661050/70‑1 (MQ=255) | CTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATTACTTAACC > W3110S.gb/2902260‑2902398 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |