| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,927,326 | G→A | 75.0% | V148M (GTG→ATG) | sdaC → | predicted serine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,927,326 | 0 | G | A | 75.0% | 97.7 / 35.0 | 56 | V148M (GTG→ATG) | sdaC | predicted serine transporter |
| Reads supporting (aligned to +/- strand): ref base G (6/8); new base A (17/25); total (23/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTGGTATT > W3110S.gb/2927260‑2927393 | aGCTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATggg < 1:952321/71‑1 (MQ=255) cTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTa > 1:225872/1‑71 (MQ=255) cATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGGTCCTGATCGTGGGTatg < 1:2167080/70‑1 (MQ=255) cATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTatg < 1:1313259/70‑1 (MQ=255) tGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTatgatg < 1:755013/71‑1 (MQ=255) tGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTatgatg < 1:2402462/71‑1 (MQ=255) aCCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTataat < 1:2751015/64‑1 (MQ=255) aCCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGAcc < 1:2519572/68‑1 (MQ=255) aGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGtt > 1:1314964/1‑71 (MQ=255) cttggTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg < 1:2716639/68‑1 (MQ=255) tGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCg > 1:927733/1‑70 (MQ=255) gTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTc < 1:1232222/71‑1 (MQ=255) tGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTat < 1:2753073/49‑1 (MQ=255) gACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGT‑‑‑ATGACCATCGTTCGCTTCGGTGa > 1:897896/1‑69 (MQ=255) aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTAtgatga > 1:484862/1‑52 (MQ=255) aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGt > 1:2600631/1‑69 (MQ=255) aCGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGt > 1:1968794/1‑69 (MQ=255) cGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAg < 1:514550/71‑1 (MQ=255) ccACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCa < 1:2513670/71‑1 (MQ=255) cACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCa < 1:2568081/70‑1 (MQ=255) cACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAg < 1:2053281/71‑1 (MQ=255) cACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAg < 1:188783/71‑1 (MQ=255) cACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAg < 1:2566203/71‑1 (MQ=255) aCCGCCGCGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAg > 1:2749324/1‑67 (MQ=255) cgccgcGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGa > 1:1963349/1‑64 (MQ=255) cgccgcGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgatg < 1:1544852/71‑1 (MQ=255) cgccgcGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgatg < 1:1310186/71‑1 (MQ=255) cgccgcGTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAgatg < 1:1776652/71‑1 (MQ=255) gcgTGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCg > 1:251608/1‑71 (MQ=255) tGCGATTCTGTCGCTGATCCTGATCATGGGTatgatg < 1:989871/37‑1 (MQ=255) tGCGATTCTGTCGCTGATCCTGATCATGGGTATGATGAc > 1:2612297/1‑39 (MQ=255) aTTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGc < 1:1950742/71‑1 (MQ=255) aTTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGc < 1:524721/71‑1 (MQ=255) aTTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGc < 1:1883971/71‑1 (MQ=255) aTTCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGc < 1:405131/71‑1 (MQ=255) ttCTGTCGCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg < 1:641454/71‑1 (MQ=255) tCTGTAGCTGATCCTGAACATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGa > 1:2619639/1‑71 (MQ=255) gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGa > 1:1509838/1‑68 (MQ=255) gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGa > 1:248350/1‑68 (MQ=255) gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTa < 1:930027/71‑1 (MQ=255) gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTa < 1:918578/71‑1 (MQ=255) gCTGATCCTGATCATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTa > 1:1594405/1‑71 (MQ=255) atcctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAg < 1:2414537/65‑1 (MQ=255) cctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCg > 1:2135092/1‑58 (MQ=255) cctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGa > 1:2188466/1‑62 (MQ=255) cctgatcGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg > 1:863375/1‑71 (MQ=255) cctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTAtt > 1:2014569/1‑67 (MQ=255) cctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg > 1:1867893/1‑71 (MQ=255) ctgatcATGGGTATGATGACCATCGTTCTCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg < 1:356030/70‑1 (MQ=255) ctgatcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTgg > 1:1995596/1‑70 (MQ=255) atcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCa < 1:756860/38‑1 (MQ=255) atcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCa < 1:1477453/38‑1 (MQ=255) atcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTGGTa < 1:432788/69‑1 (MQ=255) atcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTGGTa < 1:2431369/69‑1 (MQ=255) atcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTGGTAtt > 1:239399/1‑71 (MQ=255) atcATGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTGGTAtt > 1:504611/1‑71 (MQ=255) | AGCTTCATGTCTCACCAGCTGGGTATGACGCCACCGCCGCGTGCGATTCTGTCGCTGATCCTGATCGTGGGTATGATGACCATCGTTCGCTTCGGTGAGCAGATGATCGTTAAAGCGATGAGTATTCTGGTATT > W3110S.gb/2927260‑2927393 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |