| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,968,686 | A→G | 100% | I123M (ATA→ATG) | mutH → | methyl‑directed mismatch repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,968,686 | 0 | A | G | 82.2% | 63.6 / 7.8 | 28 | I123M (ATA→ATG) | mutH | methyl‑directed mismatch repair protein |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base G (15/8); total (20/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACGGGCAATAGCGGGGTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATACCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGTTGGATCACCGTTGCTGTGGAGCC > W3110S.gb/2968617‑2968753 | aaCGGGCAATAGCGGGGTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATAc > 1:1567814/1‑71 (MQ=255) aTAGCGGGGTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTg < 1:2591661/69‑1 (MQ=255) aGCGGGGTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAgg > 1:581701/1‑71 (MQ=255) aGCGGGGTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAgg > 1:2109686/1‑71 (MQ=255) aGCGGGGTGACATGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATACCGGTTGAAgg > 1:2679135/1‑71 (MQ=255) aGCGGCGTGACCTGGGTAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAg > 1:1268075/1‑70 (MQ=255) gCGGGGTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAgg > 1:723533/1‑70 (MQ=255) gCGGGGTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAg > 1:2027541/1‑69 (MQ=255) gCGGGGTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAGGc > 1:2033745/1‑71 (MQ=255) aCCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAGGCGAGCgcag < 1:40946/71‑1 (MQ=255) aCCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATACCGGTTGAAGGCGAGCGCAGCATCCCGCt > 1:1851891/1‑71 (MQ=255) ccAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGa < 1:829233/49‑1 (MQ=255) ccAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAGGCGAGCGCAGCATCCCGCTg > 1:1677690/1‑71 (MQ=255) ccACGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAGGCGAGCGCAGCATCCCGCTGgcgc < 1:2261901/71‑1 (MQ=255) cGTGCGCCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGc > 1:1638054/1‑71 (MQ=255) gcgcCACAAACTCAAACGCGTACTGTGGATGCCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTc > 1:1087948/1‑71 (MQ=255) cacaAACTCAAACGCGTACTGTGGATGCCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGt > 1:914552/1‑71 (MQ=255) caAACTCAAACGCGTACTGTGGATGCCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGTTg > 1:1911348/1‑71 (MQ=255) caAACTCAAACGCGTACTGTGGATGCCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGTTg > 1:2729326/1‑71 (MQ=255) aaaCTCAAACGCGTACTGTGGATGCCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGTTgg < 1:2602291/71‑1 (MQ=255) aaaCTCAAACGCGTACTGTGGATGCCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGTTgg < 1:2621648/71‑1 (MQ=255) aaaCTCAAACGCGTACTGTGGATGCCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGTTgg < 1:515289/71‑1 (MQ=255) aaCTCAAACGCGTACTGTGGATACCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGTTGGa > 1:569272/1‑71 (MQ=255) cTCAAACGCGTACTGTGGATACCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGTTGGa > 1:505795/1‑69 (MQ=255) cAAACGCGTACTGTGGATGCCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGTTGGATCAc > 1:1771255/1‑71 (MQ=255) cgTACTGTGGATGCCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGt < 1:2188932/57‑1 (MQ=255) gtgGATGCCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGTTGGATCACCGTTGCtgtg > 1:1601111/1‑69 (MQ=255) gATGCCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGTTGGATCACCGTTGCTGTGGAGcc > 1:2538918/1‑71 (MQ=255) aCCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGt > 1:181654/1‑45 (MQ=255) aCCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGt > 1:178322/1‑45 (MQ=255) | AACGGGCAATAGCGGGGTGACCTGGGAAACCAGCCACGTGCGCCACAAACTCAAACGCGTACTGTGGATACCGGTTGAAGGCGAGCGCAGCATCCCGCTGGCGCAGCGTCGCGTTGGATCACCGTTGCTGTGGAGCC > W3110S.gb/2968617‑2968753 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |