| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,971,401 | A→T | 56.2% | V373E (GTG→GAG) | ygeD ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,971,401 | 0 | A | T | 56.2% | 19.9 / 45.6 | 41 | V373E (GTG→GAG) | ygeD | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (13/5); new base T (14/9); total (27/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTG > W3110S.gb/2971341‑2971463 | cAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt < 1:457446/71‑1 (MQ=255)cAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGAt < 1:454323/71‑1 (MQ=255) aTCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCgg < 1:643596/65‑1 (MQ=255) tCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGa > 1:1997118/1‑66 (MQ=255) ccACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTa > 1:47836/1‑71 (MQ=255) ccACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGa > 1:760530/1‑65 (MQ=255) ccACAGCGCCGTTATTGCCAGCGCAAACAGCGAACCGAAGCCAATGCCAATGGGCACGACCGGGa > 1:1465853/1‑65 (MQ=255) gcgcCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCAtt < 1:737915/71‑1 (MQ=255) gCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCa > 1:791500/1‑67 (MQ=255) gCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCa > 1:705005/1‑67 (MQ=255) ccAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAg > 1:948435/1‑71 (MQ=255) ccAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAg > 1:1538532/1‑71 (MQ=255) aGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTa < 1:1091755/71‑1 (MQ=255) aGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTa < 1:2267498/71‑1 (MQ=255) aGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCAt > 1:805552/1‑47 (MQ=255) aGCGCACCGAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCAt > 1:804316/1‑47 (MQ=255) gcACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCAt > 1:1054332/1‑71 (MQ=255) gAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCa > 1:39134/1‑62 (MQ=255) gAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCa > 1:335640/1‑62 (MQ=255) gAAGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATCCCCa > 1:1670706/1‑62 (MQ=255) aaGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaacaa < 1:2570126/71‑1 (MQ=255) aaGCCAATGCCAATGGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATcaacaa < 1:2593413/71‑1 (MQ=255) atgccaatgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGt < 1:217414/46‑1 (MQ=255) gccaatgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcgc > 1:562885/1‑71 (MQ=255) gccaatgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcgc > 1:559748/1‑71 (MQ=255) gccaatgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcgc > 1:2208355/1‑71 (MQ=255) gccaatgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTa < 1:1355415/45‑1 (MQ=255) gccaatgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGgcgc > 1:1727839/1‑71 (MQ=255) ccaatgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAacaaca > 1:2484700/1‑64 (MQ=255) ccaatgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCt > 1:1387058/1‑71 (MQ=255) ccaatgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCt < 1:1224618/71‑1 (MQ=255) ccaatgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCt > 1:1957077/1‑71 (MQ=255) ccaatgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCt > 1:2551383/1‑71 (MQ=255) ccaatgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCt > 1:2175473/1‑71 (MQ=255) ccaatgGGCACGACCGGGATACCTATCATTACCGCCAGGGAGTAAATGCCCAGCATCAACAACATGGCGCt > 1:2636925/1‑71 (MQ=255) caatgGGCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTg < 1:239194/71‑1 (MQ=255) caatgGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTa < 1:1792140/43‑1 (MQ=255) gggCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTaaa > 1:2109283/1‑41 (MQ=255) gggCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTaaa > 1:296906/1‑41 (MQ=255) gggCTCGACCGGGATGCCTATCATTACCGCCAGCGAGTaaa > 1:2112490/1‑41 (MQ=255) gggCACGACCGGGATGCCTATCATTACCGCCAGCGAGTa < 1:926827/39‑1 (MQ=255) aCGACCGGGATGCCTATCATTACCGCCAGCGAGTa < 1:74084/35‑1 (MQ=255) | CAGATCCACAGCGCCGTTATTGCCAGCGCAAACAGCGCACCGAAGCCAATGCCAATGGGCACGACCGGGATGCCTATCATTACCGCCAGCGAGTAAATGCCCAGCATCAACAACATGGCGCTG > W3110S.gb/2971341‑2971463 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |