| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,694 | A→G | 100% | pseudogene (114/261 nt) pseudogene (114/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,694 | 0 | A | G | 100.0% | 45.3 / NA | 16 | pseudogene (114/261 nt) pseudogene (114/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (10/6); total (10/6) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAA > W3110S.gb/547627‑547756 | ttCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGc < 1:1805067/71‑1 (MQ=255) cgtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGc > 1:142814/1‑69 (MQ=255) gcgcTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAaca < 1:903671/71‑1 (MQ=255) cTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTg < 1:585284/71‑1 (MQ=255) gCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgc > 1:710784/1‑71 (MQ=255) aCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCt > 1:1022148/1‑71 (MQ=255) cttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga < 1:2083228/70‑1 (MQ=255) tcACTTATCAGCGAACTAAACGAGGGCAAAACCCTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgaga > 1:499273/1‑70 (MQ=255) tcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGgaga > 1:257582/1‑70 (MQ=255) cACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGAt > 1:2290750/1‑70 (MQ=255) ttATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGga < 1:596865/64‑1 (MQ=255) tATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc > 1:1450604/1‑44 (MQ=255) aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc > 1:2354884/1‑43 (MQ=255) aTCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc > 1:2535941/1‑43 (MQ=255) cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGaa < 1:1561665/71‑1 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATgc > 1:1904715/1‑40 (MQ=255) | TTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAA > W3110S.gb/547627‑547756 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |