| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,976,020 | C→T | 59.4% | L256F (CTC→TTC) | galR → | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,976,020 | 0 | C | T | 59.4% | 14.1 / 36.3 | 32 | L256F (CTC→TTC) | galR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (6/7); new base T (7/12); total (13/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.43e-01 | |||||||||||
GACGAGGAAGAAATTTCACTGCGGTAGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACG > W3110S.gb/2975952‑2976090 | gACGAGGAAGAAATTTCACTGCGGTAGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTtctt < 1:1427890/71‑2 (MQ=255) aCGAGGAAGAAATTTCACTGCGGTAGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCa < 1:592092/71‑1 (MQ=255) gaagaaATTTCACTGCGGTAGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTtctc < 1:1956317/65‑1 (MQ=255) aTTTCACTGCGGTAGCCTGTTATAACGATTCAATGGCGGCGTGTGCGATGGGCGTTTTCAATGATAATgg > 1:2759429/1‑70 (MQ=255) aTTTCACTGCGGTAGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATgg > 1:2630362/1‑70 (MQ=255) tCACTGCGGTAGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTAtt > 1:758067/1‑71 (MQ=255) tCACTGCGGTAGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTAtt > 1:754817/1‑71 (MQ=255) cACTGCGGTAGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTAtt < 1:2564899/70‑1 (MQ=255) aGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCgg > 1:596035/1‑71 (MQ=255) ttATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTATTGATGTACCGGGTga < 1:1381148/69‑1 (MQ=255) ataACGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTATTGATGTa > 1:2264783/1‑59 (MQ=255) aaCGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAAt < 1:16990/39‑1 (MQ=255) cGATTCAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGt < 1:1504643/71‑1 (MQ=255) gATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATaa > 1:1026863/1‑41 (MQ=255) gATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGtt < 1:2373586/71‑1 (MQ=255) aTTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATgg < 1:2150466/43‑1 (MQ=255) tCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAAt > 1:1697190/1‑71 (MQ=255) tCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAAt > 1:2382638/1‑71 (MQ=255) cAATGGCGGCGGGTGCGATGGGCGTTTTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAAt > 1:980899/1‑70 (MQ=255) cggcggGTGCGATGGGCGTTTTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCttt < 1:2575609/71‑1 (MQ=255) cggGTGCGATGGGCGTTTTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTtgat < 1:1014062/71‑1 (MQ=255) cggGTGCGATGGGCGTTTTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTtgat < 1:959805/71‑1 (MQ=255) cggGTGCGATGGGCGTTTTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTtgat < 1:918640/71‑1 (MQ=255) cggGTGCGATGGGCGTTTTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTtgat < 1:1122561/71‑1 (MQ=255) gggTGCGATGGGCGTTTTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTtgat < 1:2331589/70‑1 (MQ=255) gggTGCGATGGGCGTTTTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTtgat < 1:1098064/70‑1 (MQ=255) gggTGCGATGGGCGTTTTCAATGATAATGGTATTGATGTACCGGGAGAGATTTCGTTAATTGGCTTtgatg < 1:707897/71‑1 (MQ=255) gggTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTtgat < 1:1484050/70‑1 (MQ=255) gTGCGATGGGCGTTTTCAATGATAATGGTATTGATGTACCGGGTga < 1:503633/46‑1 (MQ=255) gTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGtt < 1:1279458/55‑1 (MQ=255) tGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTtgatga < 1:536633/69‑1 (MQ=255) gCGATGGGCGTTTTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAAt > 1:172032/1‑56 (MQ=255) gCGATGGGAGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGAttt > 1:1980371/1‑49 (MQ=255) cGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGatgatg > 1:330601/1‑69 (MQ=255) ctcAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACg > 1:143164/1‑71 (MQ=255) ctcAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACg > 1:2545795/1‑71 (MQ=255) ctcAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACg > 1:2494455/1‑71 (MQ=255) | GACGAGGAAGAAATTTCACTGCGGTAGCCTGTTATAACGATTCAATGGCGGCGGGTGCGATGGGCGTTCTCAATGATAATGGTATTGATGTACCGGGTGAGATTTCGTTAATTGGCTTTGATGATGTGCTGGTGTCACG > W3110S.gb/2975952‑2976090 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |