| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,983,818 | A→G | 9.8% | V144A (GTG→GCG) | yqeF ← | predicted acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,983,818 | 0 | A | G | 9.8% | 182.8 / 11.7 | 71 | V144A (GTG→GCG) | yqeF | predicted acyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (39/25); new base G (4/3); total (43/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
TTCGCGAGCCAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATGTG > W3110S.gb/2983748‑2983884 | ttCGCGAGCCAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGca > 1:1398227/1‑71 (MQ=255) aGCCAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGa < 1:1480188/70‑1 (MQ=255) gCCAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACaa < 1:1440232/67‑1 (MQ=255) ccAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTg < 1:582259/71‑1 (MQ=255) cAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGt > 1:2275404/1‑71 (MQ=255) cAGATTTTCGGCGGTGACACAAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGt > 1:1607417/1‑71 (MQ=255) aGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc < 1:1052748/71‑1 (MQ=255) aGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc < 1:2760610/71‑1 (MQ=255) aGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc < 1:1339487/71‑1 (MQ=255) gATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc > 1:1914813/1‑70 (MQ=255) aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaa > 1:642768/1‑71 (MQ=255) ttttCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGa > 1:275269/1‑63 (MQ=255) ttCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacc > 1:712122/1‑70 (MQ=255) gTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCt < 1:1316335/71‑1 (MQ=255) gTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCt < 1:26124/71‑1 (MQ=255) gACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTa < 1:970653/70‑1 (MQ=255) gACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTAt > 1:496521/1‑71 (MQ=255) acacCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTAtt > 1:2287537/1‑71 (MQ=255) acacCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTAtt > 1:2281078/1‑71 (MQ=255) acacCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTAtt > 1:2231347/1‑71 (MQ=255) aaTATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTg > 1:2579832/1‑49 (MQ=255) aaTATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTg > 1:372913/1‑49 (MQ=255) aaTATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTg > 1:443315/1‑49 (MQ=255) aaTATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGcc > 1:169575/1‑69 (MQ=255) aaTATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGcc > 1:1082912/1‑69 (MQ=255) aaTATGATAATCATTGAAGGCATCCCACAAACCATCATGCACAAGa > 1:2628424/1‑46 (MQ=255) tatGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGACTg < 1:1931666/47‑1 (MQ=255) atGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTg > 1:2010761/1‑64 (MQ=255) atGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTg > 1:1265554/1‑64 (MQ=255) atGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTg > 1:1414914/1‑64 (MQ=255) tgttAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCtg > 1:2800770/4‑71 (MQ=255) tGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCtg < 1:2598768/71‑1 (MQ=255) gATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCtg < 1:1255025/70‑1 (MQ=255) aTAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaaccaa > 1:186413/1‑51 (MQ=255) aaTCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTAt > 1:574083/1‑57 (MQ=255) tCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacc < 1:472686/45‑1 (MQ=255) tCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacc < 1:761389/45‑1 (MQ=255) tCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacc < 1:764603/45‑1 (MQ=255) tCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacc < 1:1775040/45‑1 (MQ=255) tCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGc < 1:704543/52‑1 (MQ=255) tCATTGAAGGCATCCCACAACCCATCATACACAAGACTGTCAACCAACTGGc < 1:312464/52‑1 (MQ=255) cATTGAAGGCATCCCACAACCCATCATGCACAAGa > 1:129755/1‑35 (MQ=255) cATTGAAGGCATCCCACAACCCATCATGCACAAGa > 1:618378/1‑35 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaa > 1:1499552/1‑41 (MQ=255) ttGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacc < 1:1479516/42‑1 (MQ=255) tGAAGGCATCCCACAACCCATCATGCACAAGACTGTca > 1:377737/1‑38 (MQ=255) tGAAGGCATCCCACAACCCATCATGCACAAGACTGTca > 1:2207306/1‑38 (MQ=255) gAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGc > 1:2377358/1‑71 (MQ=255) aTCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCg < 1:2278808/66‑1 (MQ=255) aTCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAt < 1:722693/71‑1 (MQ=255) aTCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAt < 1:2808898/71‑1 (MQ=255) cccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTAt > 1:1874067/1‑42 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTa > 1:904711/1‑40 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcag > 1:918228/1‑71 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcag > 1:517125/1‑71 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcag > 1:920993/1‑71 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcag > 1:895737/1‑71 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcag > 1:393529/1‑71 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcag > 1:743411/1‑71 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtca > 1:515452/1‑70 (MQ=255) cacaACCCATCATGCACAAGACTGTCAACCAACTGGc < 1:1979623/37‑1 (MQ=255) caACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcagtca < 1:2269182/71‑1 (MQ=255) caACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcagt > 1:2749412/1‑69 (MQ=255) aCCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcagtcag > 1:2627641/1‑70 (MQ=255) tcatGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCtg < 1:801004/41‑1 (MQ=255) atGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgtg > 1:1046482/1‑71 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCtgtg > 1:2173691/1‑41 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCAc > 1:1307600/1‑44 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgtg > 1:1902365/1‑71 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgt < 1:1789317/70‑1 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgt < 1:639990/70‑1 (MQ=255) tGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgtg < 1:2155058/70‑1 (MQ=255) cacaAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCAcc < 1:733097/42‑1 (MQ=255) cacaAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCAcc < 1:1667978/42‑1 (MQ=255) cacaAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGaa > 1:2259985/1‑62 (MQ=255) | TTCGCGAGCCAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATGTG > W3110S.gb/2983748‑2983884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |