Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,989,682 C→T 7.7% intergenic (+26/‑242) ygeF → / → ygeG hypothetical protein/predicted chaperone

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,989,6820CT 7.7% 198.0 / 5.7 65intergenic (+26/‑242)ygeF/ygeGhypothetical protein/predicted chaperone
Reads supporting (aligned to +/- strand):  ref base C (29/31);  new base T (3/2);  total (32/33)
Fisher's exact test for biased strand distribution p-value = 6.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

CGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGTGAATATTAGTAA  >  W3110S.gb/2989612‑2989751
                                                                       |                                                                     
cGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATc                                                                       <  1:2252756/71‑1 (MQ=255)
 gACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATcc                                                                      <  1:788513/71‑1 (MQ=255)
       cTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAAATGGCAATTGATACTATGAATCCAGGt                                                                  >  1:1171231/1‑70 (MQ=255)
       cTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCa                                                                >  1:1762849/1‑71 (MQ=255)
       cTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCa                                                                >  1:768573/1‑71 (MQ=255)
        tACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCa                                                                >  1:559361/1‑70 (MQ=255)
          cccTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCATGTCATa                                                              <  1:2670651/70‑1 (MQ=255)
          cccTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATa                                                              <  1:1026888/70‑1 (MQ=255)
          cccTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATa                                                              <  1:1663728/70‑1 (MQ=255)
           ccTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATaca                                                            <  1:598206/71‑1 (MQ=255)
            cTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAg                                                                    >  1:1424269/1‑62 (MQ=255)
               ctcAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACAt                                                         >  1:2501856/1‑70 (MQ=255)
                tcAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATaca                                                            <  1:2763859/66‑1 (MQ=255)
                tcAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACAt                                                         >  1:239119/1‑69 (MQ=255)
                       tCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCAtt                                                <  1:797121/71‑1 (MQ=255)
                            tttttAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAgg                                                    <  1:1330098/62‑1 (MQ=255)
                              tttAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACAGATTAAGGCATTTATg                                            <  1:952980/68‑1 (MQ=255)
                              tttAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGt                                           <  1:545244/69‑1 (MQ=255)
                                     ttCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAg                                                     >  1:2016846/1‑52 (MQ=255)
                                     ttCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGt                                   <  1:21759/70‑1 (MQ=255)
                                              aaaaaTGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGAt                          <  1:790297/71‑1 (MQ=255)
                                               aaaaTGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATa                         <  1:1523314/71‑1 (MQ=255)
                                                  aTGGCAATTGATACTATGAATTCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATaa                        >  1:1394838/1‑69 (MQ=255)
                                                  aTGGCAATTGATACTATGAATTCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAAc                       >  1:1459972/1‑70 (MQ=255)
                                                  aTGGCAATTGATACTATGAATTCAGGTCATACACATTAAGGCATTAATGTAATGTCGTAATTAAGATAAc                       >  1:525361/1‑70 (MQ=255)
                                                  aTGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAAc                       >  1:1394420/1‑70 (MQ=255)
                                                       aaTTGATACTATGAATCCAGGTCATACACATTAAGGCAtt                                                <  1:1251063/40‑1 (MQ=255)
                                                        aTTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATg                                            <  1:2203128/43‑1 (MQ=255)
                                                        aTTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATg                                            <  1:1144429/43‑1 (MQ=255)
                                                        aTTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATg                                            <  1:1216438/43‑1 (MQ=255)
                                                        aTTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATg                                            <  1:1707812/43‑1 (MQ=255)
                                                        aTTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGAt                          <  1:2348417/61‑1 (MQ=255)
                                                         ttGATACTATGAATTCAGGTCATACACATTAAGGCATTTATGt                                           <  1:2224858/43‑1 (MQ=255)
                                                         ttGATACTATGAATTCAGGTCATACACATTAAGGCATTTATGt                                           <  1:2063798/43‑1 (MQ=255)
                                                         ttGATACTATGAATCCAGGTCATACACATTAAGGCAtt                                                <  1:44945/38‑1 (MQ=255)
                                                         ttGATACTATGAATCCAGGTCATACACATTAAGGCAtt                                                <  1:2384182/38‑1 (MQ=255)
                                                         ttGATACTATGAATCCAGGTCATACACATTAAGGCATTTa                                              >  1:2435812/1‑40 (MQ=255)
                                                         ttGATACTATGAATCCAGGTCATACACATTAAGGCATTTa                                              >  1:1136053/1‑40 (MQ=255)
                                                         ttGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGAt                          <  1:2283102/60‑1 (MQ=255)
                                                          tGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAg                            >  1:2778460/1‑57 (MQ=255)
                                                          tGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATaa                        >  1:495761/1‑61 (MQ=255)
                                                          tGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGt              >  1:1330311/1‑71 (MQ=255)
                                                          tGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGt              >  1:746740/1‑71 (MQ=255)
                                                          tGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGt              >  1:604556/1‑71 (MQ=255)
                                                           gATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTaa                                         >  1:72377/1‑43 (MQ=255)
                                                           gATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAg                            >  1:2340755/1‑56 (MQ=255)
                                                           gATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATaa                        >  1:783666/1‑60 (MQ=255)
                                                           gATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGt              >  1:1829537/1‑70 (MQ=255)
                                                           gATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGt              >  1:305905/1‑70 (MQ=255)
                                                             tACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGTGaa           <  1:1815115/71‑1 (MQ=255)
                                                             tACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGTGaa           <  1:2098405/71‑1 (MQ=255)
                                                             tACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGTGaa           <  1:2548043/71‑1 (MQ=255)
                                                              aCTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTaa                                 >  1:1741290/1‑48 (MQ=255)
                                                               cTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACtaataa                 <  1:1177443/63‑1 (MQ=255)
                                                               cTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACtaa                    <  1:476324/60‑1 (MQ=255)
                                                               cTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGTGAata         >  1:2083130/1‑71 (MQ=255)
                                                                tATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTaa                             <  1:1746854/50‑1 (MQ=255)
                                                                 aTGAATCCAGGTCATACACATTAAGGCATTTATGTAAt                                        >  1:2274375/1‑38 (MQ=255)
                                                                 aTGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATaa                        >  1:1711011/1‑54 (MQ=255)
                                                                 aTGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACtaata                  >  1:1794382/1‑60 (MQ=255)
                                                                 aTGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGTGAatat        >  1:1512670/1‑70 (MQ=255)
                                                                  tGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTaa                             <  1:375301/48‑1 (MQ=255)
                                                                  tGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTaa                             <  1:2317219/48‑1 (MQ=255)
                                                                  tGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGAt                          <  1:1528039/51‑1 (MQ=255)
                                                                  tGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGTGAatat        >  1:2513457/1‑69 (MQ=255)
                                                                   gAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGTGAATATTAg     >  1:27025/1‑71 (MQ=255)
                                                                      tCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGTGAATATTAGTaa  <  1:2598647/71‑1 (MQ=255)
                                                                       |                                                                     
CGACATTCTACCCTTCTCAAAAATCCCCTTTTTAATTTTCATTGAAAAAATGGCAATTGATACTATGAATCCAGGTCATACACATTAAGGCATTTATGTAATGTCGTAATTAAGATAACTAATAAGGTGAATATTAGTAA  >  W3110S.gb/2989612‑2989751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: