| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,989,906 | A→G | 8.1% | intergenic (+250/‑18) | ygeF → / → ygeG | hypothetical protein/predicted chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,989,906 | 0 | A | G | 8.1% | 159.0 / 6.7 | 62 | intergenic (+250/‑18) | ygeF/ygeG | hypothetical protein/predicted chaperone |
| Reads supporting (aligned to +/- strand): ref base A (34/23); new base G (2/3); total (36/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
CTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCA > W3110S.gb/2989836‑2989971 | cTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGaca < 1:978029/71‑1 (MQ=255) aTAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGacac < 1:2624044/69‑1 (MQ=255) aTAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACAt < 1:1681047/71‑1 (MQ=255) aTTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATca > 1:2677340/1‑70 (MQ=255) tttAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACGCATcaca < 1:1983744/71‑1 (MQ=255) tttAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATcaca < 1:1119799/71‑1 (MQ=255) aaaGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGaa < 1:1501426/71‑1 (MQ=255) aaGATAAATATAAAAATATCAATGAGTCAATCTATAATAATTTGGCATCACGAGACACATCACAGAGGaa < 1:619401/70‑1 (MQ=255) aaGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGaa > 1:2645135/1‑70 (MQ=255) gATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAatat < 1:27154/71‑1 (MQ=255) gATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAatat < 1:1912563/71‑1 (MQ=255) aTAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAatat > 1:591958/1‑70 (MQ=255) tataAAAATATCAATGAGTCAATCAATAATATTTTGGCATCACGAGACACATcac < 1:2249862/55‑1 (MQ=255) ataAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACag > 1:1449042/1‑56 (MQ=255) taAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGc > 1:193137/1‑70 (MQ=255) aaaaTATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAatat > 1:900848/1‑61 (MQ=255) tatCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACAt < 1:589461/44‑1 (MQ=255) tatCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATg < 1:2763905/61‑1 (MQ=255) tatCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATg < 1:2130279/61‑1 (MQ=255) tCAATGAGTCAATCAATAATAATTTGGCATCACGAGacac < 1:1843725/40‑1 (MQ=255) tCAATGAGTCAATCAATAATAATTTGGCATCACGAGacac < 1:1151983/40‑1 (MQ=255) tCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAg > 1:1319493/1‑61 (MQ=255) tCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAAc > 1:1462383/1‑70 (MQ=255) aaTGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACaa < 1:1378677/70‑1 (MQ=255) gAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTg > 1:1293979/1‑70 (MQ=255) gAGTCAATCAATAATAATTTGGCATAACGAGACACATcac > 1:2595386/1‑40 (MQ=255) aGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAAtt > 1:21710/1‑68 (MQ=255) aGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGa > 1:1590126/1‑70 (MQ=255) aGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGa < 1:2668621/70‑1 (MQ=255) aGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGa > 1:805525/1‑70 (MQ=255) gTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGaaa < 1:2678225/71‑1 (MQ=255) gTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGaaa < 1:729195/71‑1 (MQ=255) aatcaatAATAATTTGGCATCACGAGACACATcac > 1:2563536/1‑35 (MQ=255) aatcaatAATAATTTGGCATCACGAGACACATCACAGAGGaa > 1:880127/1‑42 (MQ=255) aatcaatAATAATTTGGCATCACGAGACACATCACAGAGGaa > 1:2038760/1‑42 (MQ=255) tcaatAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACaa > 1:302019/1‑60 (MQ=255) tcaatAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACaa > 1:2345611/1‑60 (MQ=255) tcaatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTc < 1:2125600/71‑1 (MQ=255) caatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTc > 1:1521907/1‑70 (MQ=255) caatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATAAGCACAGAAACAATTGAAATAtt > 1:2237598/1‑69 (MQ=255) aataataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAAtt > 1:1951688/1‑60 (MQ=255) aataataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaa > 1:2364026/1‑71 (MQ=255) aataataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaa > 1:1378263/1‑71 (MQ=255) aataataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaa > 1:553100/1‑71 (MQ=255) aataataatTTGGCATCACGAGACACAT‑‑CAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaat > 1:155814/1‑70 (MQ=255) ataataatTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaat < 1:689509/71‑1 (MQ=255) ataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGcaca > 1:235504/1‑47 (MQ=255) ataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaataa > 1:2609937/1‑70 (MQ=255) ataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaataa > 1:2212336/1‑70 (MQ=255) taatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGa < 1:1855355/57‑1 (MQ=255) atTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGt < 1:2716460/71‑1 (MQ=255) ttGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgg > 1:867111/1‑70 (MQ=255) tGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgat > 1:1150084/1‑71 (MQ=255) gCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACa < 1:2008697/45‑1 (MQ=255) gCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACa < 1:2704891/45‑1 (MQ=255) gCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgatg < 1:485661/70‑1 (MQ=255) gCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgatg < 1:2305192/70‑1 (MQ=255) gCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgatg < 1:2295848/70‑1 (MQ=255) tCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaat < 1:654596/57‑1 (MQ=255) aCGAGACACATCACAGAGGAATATTATGAGCACAGAAACa > 1:13705/1‑40 (MQ=255) aCGAGACACATCACAGAGGAATATTATGAGCACAGAAACAAtt > 1:869065/1‑43 (MQ=255) aCGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAata > 1:770275/1‑49 (MQ=255) aCGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGaa > 1:810306/1‑67 (MQ=255) aCGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGaa > 1:1967334/1‑67 (MQ=255) aCGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATggg > 1:792731/1‑71 (MQ=255) gagaCACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCa < 1:1940298/71‑1 (MQ=255) | CTCATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCA > W3110S.gb/2989836‑2989971 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |