| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,993,568 | C→A | 16.6% | intergenic (‑9/+25) | ygeK ← / ← ygeL | predicted DNA‑binding transcriptional regulator/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,993,568 | 0 | C | A | 16.6% | 83.8 / 4.2 | 30 | intergenic (‑9/+25) | ygeK/ygeL | predicted DNA‑binding transcriptional regulator/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (9/16); new base A (2/3); total (11/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
ATATGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTTTCTTCAGA > W3110S.gb/2993507‑2993635 | atatGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATtc > 1:890863/1‑71 (MQ=255) tctatctTATGCGATTTAACCCATTTTACGAGTGCGGCCCCCATCATTCCACCTGCaaa < 1:1847048/59‑1 (MQ=255) tatctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata > 1:2720015/1‑70 (MQ=255) tctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata < 1:702829/68‑1 (MQ=255) tctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata < 1:333520/68‑1 (MQ=255) tctTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata < 1:2738795/68‑1 (MQ=255) ttATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTa > 1:448684/1‑61 (MQ=255) aTGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATAtt < 1:2270332/70‑1 (MQ=255) aTGCGATTTAACCCATTTTACGAGTTCCGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATAtt < 1:1166619/70‑1 (MQ=255) aTTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACATGCaaa < 1:510130/46‑1 (MQ=255) ttAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTg > 1:2120566/1‑70 (MQ=255) aCCCATTTTACGAGTTCGGCCCCCATCATTCCACATGCAAATTCTCCTAAAATAAATATTTAGGCTGTAg < 1:1271878/70‑1 (MQ=255) ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAaaata < 1:838473/49‑1 (MQ=255) ttttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGt < 1:1631846/71‑1 (MQ=255) ttttACGAGTTCGGCCCCCATCATTCCACATGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGt < 1:11707/71‑1 (MQ=255) ttACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTa > 1:138100/1‑71 (MQ=255) tACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTa < 1:1835288/70‑1 (MQ=255) aCGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTac < 1:556017/70‑1 (MQ=255) aGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTaa < 1:573675/38‑1 (MQ=255) ccATCATTCCACCTGCAAATTCTCCTAaaataaat < 1:1928233/35‑1 (MQ=255) ccATCATTCCACCTGCAAATTCTCCTAaaataaat < 1:1837663/35‑1 (MQ=255) atcatTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGAt > 1:2477255/1‑48 (MQ=255) atcatTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGt < 1:202981/52‑1 (MQ=255) tcatTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTa < 1:580051/53‑1 (MQ=255) atTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTg > 1:2220003/1‑48 (MQ=255) atTCCACATGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTttctt > 1:450004/1‑71 (MQ=255) atTCCACATGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTttctt > 1:675493/1‑71 (MQ=255) ccACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTttctt > 1:2383659/1‑68 (MQ=255) ccACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTttctt > 1:137628/1‑68 (MQ=255) cACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTTTCTTCAGa < 1:1995714/71‑1 (MQ=255) | ATATGAGCATCTATCTTATGCGATTTAACCCATTTTACGAGTTCGGCCCCCATCATTCCACCTGCAAATTCTCCTAAAATAAATATTTAGGCTGTAGATTTGTTACACTCCGCTATAGCTTTCTTCAGA > W3110S.gb/2993507‑2993635 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |