| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,018,630 | G→A | 40.0% | D272N (GAC→AAC) | ssnA → | predicted chlorohydrolase/aminohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,018,630 | 0 | G | A | 40.0% | 51.1 / 78.6 | 70 | D272N (GAC→AAC) | ssnA | predicted chlorohydrolase/aminohydrolase |
| Reads supporting (aligned to +/- strand): ref base G (25/17); new base A (19/9); total (44/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
ACGACTGGCGCAATTCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCAT > W3110S.gb/3018560‑3018698 | aCGACTGGCGCAATTCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATg < 1:501808/71‑1 (MQ=255)aCGACTGGCGCAATTCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATg < 1:1889429/71‑1 (MQ=255) gACTGGCGCAATTCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAAc < 1:474546/71‑1 (MQ=255) gcgcAATTCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCAc < 1:1426451/71‑1 (MQ=255) gcAATTCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCAccc > 1:2234726/1‑71 (MQ=255) gcAATTCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCAccc > 1:405082/1‑71 (MQ=255) cAATTCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCAccc > 1:2036258/1‑70 (MQ=255) cAATTCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCAccc > 1:152318/1‑70 (MQ=255) ttCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACAt > 1:2439459/1‑62 (MQ=255) tCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACt > 1:1809761/1‑70 (MQ=255) cGATCTCTTCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCACCCTACTc > 1:2463032/1‑70 (MQ=255) cGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGa > 1:127202/1‑57 (MQ=255) cGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGCCATCACCCTACTc > 1:2718864/1‑70 (MQ=255) cGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGAc > 1:332435/1‑58 (MQ=255) cGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTc > 1:106588/1‑70 (MQ=255) gATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCa < 1:1894135/70‑1 (MQ=255) ctcATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGAAATCACCCTACt > 1:2775907/1‑65 (MQ=255) ctcATCGACAGCAAAACGCTGGTCGCTCATGGGCAGTACTTGTCGAAAGATGACATCACCCTACt > 1:208822/1‑65 (MQ=255) aTCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGATAGATGACATCACCCTACTCAATCAgcg > 1:1361580/1‑71 (MQ=255) aTCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAgcg > 1:1454138/1‑71 (MQ=255) aTCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAgcg > 1:1641296/1‑71 (MQ=255) aTCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCa > 1:1419840/1‑55 (MQ=255) gACAGCAAAACGCTGGTCGCTCATGGGCTGTGCTTGTCGAAAGATGACa > 1:2363489/1‑49 (MQ=255) gACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGa > 1:1309952/1‑71 (MQ=255) gACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCACCCTACTCAATCAGcgcg > 1:139418/1‑70 (MQ=255) gACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCACCCTACTCAATCAGCGCGa > 1:2778625/1‑71 (MQ=255) gACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCACCCTACTCAATCAGCGCGa > 1:2642463/1‑71 (MQ=255) gACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCACCCTACTCAATCAGCGCGa > 1:1267907/1‑71 (MQ=255) gACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCACCCTACTCAATCAGCGCGa > 1:52402/1‑71 (MQ=255) cAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCACCCTACTCAATCAGCGCGATGCGt < 1:491750/71‑1 (MQ=255) aaaaCGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCACCCTACTCAATCAGCGCGATGCGt < 1:2305361/70‑1 (MQ=255) aaCGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTcc < 1:2608643/71‑1 (MQ=255) aaCGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTcc < 1:679207/71‑1 (MQ=255) aaCGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTcc < 1:1139662/71‑1 (MQ=255) aCGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCt < 1:574741/71‑1 (MQ=255) aCGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATAACATCACCCTACTCAATCAGCGCGATGCGTTCCt < 1:578424/71‑1 (MQ=255) cGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCaa > 1:2569867/1‑52 (MQ=255) tCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCAt < 1:1820612/71‑1 (MQ=255) tCGCTCATGGGCTGTACTTGTCGAAAGATAACATCACCCTACTCAATCAGCGCGATGCGt < 1:1270127/60‑1 (MQ=255) cGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGa > 1:1411216/1‑54 (MQ=255) cATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTg < 1:2000926/60‑1 (MQ=255) cATGGGCTGTACTTGTCGAAAGATAACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGc > 1:2566698/1‑71 (MQ=255) gggCTGTACTTGTCGAAAGATAACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCg > 1:912494/1‑71 (MQ=255) gggCTGTACTTGTCGAAAGATAACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCg > 1:2365108/1‑71 (MQ=255) ggCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGt > 1:1359684/1‑71 (MQ=255) ggCTGTACTTGTCGAAAGATGACATCACCCAACTCAATCAGCGCGATGCGTTCCTGGT‑‑ATAACGCCCGtt > 1:892047/1‑70 (MQ=255) ggCTGTACTTGTCGAAAGATAACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGt > 1:242893/1‑71 (MQ=255) tACTTGTCGAAAGATGACATCACCCTACTCAATCAgcgc < 1:751204/39‑1 (MQ=255) tACTTGTCGAAAGATGACATCACCCTACTCAATCAgcgc < 1:1417515/39‑1 (MQ=255) tACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTcc < 1:437403/49‑1 (MQ=255) tACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCt < 1:2510119/50‑1 (MQ=255) tACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCaaa < 1:735423/71‑1 (MQ=255) aCTTGTCGAAAGATAACATCACCCTACTCAATCAGCGCGATGCGt < 1:1173086/45‑1 (MQ=255) cTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTg > 1:1189885/1‑49 (MQ=255) cTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTg > 1:1697205/1‑49 (MQ=255) cTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACa > 1:2732303/1‑71 (MQ=255) cTTGTCGAAAGATAACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACa > 1:2249472/1‑71 (MQ=255) ttGTCGAAAGATGACATCACCCTACTCAATCAgcgc < 1:2492296/36‑1 (MQ=255) ttGTCGAAAGATAACATCACCCTACTCAATCAGCGCGATg > 1:798768/1‑40 (MQ=255) ttGTCGAAAGATAACATCACCCTACTCAATCAGCGCGATGCGt < 1:1546094/43‑1 (MQ=255) tGTCGAAAGATAACATCACCCTACTCAATCAGCGCGa > 1:2091488/1‑37 (MQ=255) gTCGAAAGATGACATCACCCTACTCAATCAGCGCGa > 1:384343/1‑36 (MQ=255) gTCGAAAGATGACATCACCCTACTCAATCAGCGCGa > 1:1749463/1‑36 (MQ=255) gTCGAAAGATGACATCACCCTACTCAATCAGCGCGa > 1:529420/1‑36 (MQ=255) gTCGAAAGATGACATCACCCTACTCAATCAGCGCGa > 1:1996877/1‑36 (MQ=255) gTCGAAAGATAACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATg > 1:2558525/1‑70 (MQ=255) gAAAGATAACATCACCCTACTCAATCAGGGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGaac < 1:72822/70‑1 (MQ=255) gAAAGATAACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATaa > 1:1920110/1‑51 (MQ=255) aaGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGaacaac < 1:340353/71‑1 (MQ=255) aaGATAACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGaacaac < 1:680633/71‑1 (MQ=255) tGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCAt < 1:192620/70‑1 (MQ=255) tGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCAt < 1:2265796/70‑1 (MQ=255) | ACGACTGGCGCAATTCGATCTCATCGACAGCAAAACGCTGGTCGCTCATGGGCTGTACTTGTCGAAAGATGACATCACCCTACTCAATCAGCGCGATGCGTTCCTGGTGCATAACGCCCGTTCAAACATGAACAACCAT > W3110S.gb/3018560‑3018698 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |