| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,065,306 | A→G | 73.5% | V175A (GTT→GCT) | ygfI ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,065,306 | 0 | A | G | 73.5% | 57.4 / 20.1 | 34 | V175A (GTT→GCT) | ygfI | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (4/5); new base G (12/13); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
TGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCA > W3110S.gb/3065246‑3065374 | tGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGcgagaagcga > 1:1151145/1‑64 (MQ=255)tGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGcc > 1:1921222/1‑71 (MQ=255)tGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGcc > 1:1714211/1‑71 (MQ=255)tGTACCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGcc > 1:1191680/1‑71 (MQ=255) gCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGc < 1:1872963/71‑1 (MQ=255) cTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGcgagaagcgag < 1:2739043/51‑1 (MQ=255) cTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTAATAACGGCGAGAAGCGAGAAAg < 1:2011730/55‑1 (MQ=255) cTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAgggt < 1:1464330/66‑4 (MQ=255) aaGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAg < 1:339161/69‑1 (MQ=255) ctcttgctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGa < 1:916025/71‑1 (MQ=255) ttgctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCt < 1:91261/43‑1 (MQ=255) ttgctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCt < 1:1960003/43‑1 (MQ=255) ttgctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTa < 1:2311236/44‑1 (MQ=255) ttgctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTa < 1:1999794/44‑1 (MQ=255) gctcttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCa > 1:1022965/1‑62 (MQ=255) ttgTCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGc < 1:1363732/46‑1 (MQ=255) gTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGtt > 1:2605707/1‑54 (MQ=255) tCGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGttt < 1:692088/70‑1 (MQ=255) cGCTCTTCATAACGGCGAGAAGCGAGAAAGCCTaa > 1:2553368/1‑35 (MQ=255) gCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTcc < 1:2543104/71‑1 (MQ=255) tctTCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGc < 1:2745113/39‑1 (MQ=255) ttCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGAt > 1:1199640/1‑54 (MQ=255) ttCATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATa < 1:423553/55‑1 (MQ=255) cATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTcc > 1:564181/1‑71 (MQ=255) cATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTcc > 1:561007/1‑71 (MQ=255) cATAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTcc > 1:2771402/1‑71 (MQ=255) cATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGt < 1:1921516/68‑1 (MQ=255) aTAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGtttt > 1:2373262/1‑62 (MQ=255) tAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGtt > 1:1844718/1‑43 (MQ=255) tAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGtt > 1:1933315/1‑43 (MQ=255) tAACGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTg < 1:2389449/71‑1 (MQ=255) tAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTg < 1:457072/71‑1 (MQ=255) aCGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCAc > 1:336390/1‑44 (MQ=255) aCGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGaa > 1:516674/1‑71 (MQ=255) aCGGCGAGAAGCGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGa < 1:12594/70‑1 (MQ=255) aCGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGtt > 1:1926207/1‑65 (MQ=255) gaagcgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGttt < 1:2463769/51‑1 (MQ=255) aaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGa > 1:1191278/1‑39 (MQ=255) aaacgagaaaGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCa < 1:2023956/71‑1 (MQ=255) | TGTAGCCAGGGCAGCTCCTGAAGCAGCTCTTGCTCTTGTCGCTCTTCATAACGGCGAGAAACGAGAAAGCCTAATCGCAGCGGTGGTAGTTCACTGATAGTCAGGTTTTCCAGTTCCTGAACTCGTGCA > W3110S.gb/3065246‑3065374 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |