| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,100,981 | A→G | 15.6% | H176H (CAT→CAC) | yggH ← | tRNA (m7G46) methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,100,981 | 0 | A | G | 15.6% | 75.8 / 8.1 | 32 | H176H (CAT→CAC) | yggH | tRNA (m7G46) methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (5/22); new base G (3/2); total (8/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.54e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
TTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTT > W3110S.gb/3100912‑3101051 | tttATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCata < 1:2530701/70‑1 (MQ=255) taACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGaa < 1:592026/71‑1 (MQ=255) taACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGaa < 1:2762769/71‑1 (MQ=255) taACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGaa < 1:2759548/71‑1 (MQ=255) taACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGaa < 1:1786157/71‑1 (MQ=255) taACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGaa < 1:1932186/71‑1 (MQ=255) taACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGaa < 1:2704041/71‑1 (MQ=255) aaCCGTCAATAGAAGACATCACTTCAAGCATATGTT‑‑GCATAAGGTTCCCAGTCGGTCGCCATATGGAATAc > 1:294549/1‑71 (MQ=255) gaagaCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTGGAATa > 1:1239163/1‑61 (MQ=255) ttCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGaa < 1:314860/48‑1 (MQ=255) ttCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGaa < 1:2209898/48‑1 (MQ=255) cATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTGGaa > 1:897718/1‑42 (MQ=255) cATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTGGaa > 1:867121/1‑42 (MQ=255) tatGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTGGaa < 1:332627/40‑1 (MQ=255) tatGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATgg < 1:1325549/38‑1 (MQ=255) tatGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATa > 1:1471554/1‑42 (MQ=255) tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAg < 1:428719/71‑1 (MQ=255) tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAg > 1:1478181/1‑71 (MQ=255) ccGCATAAGGTTCCCAGTCGGTCGCCATATGGATTACGCCCCCCAGCTGCAGTTTGCt < 1:2284212/58‑1 (MQ=255) cATAAGGTTCCCAGTCGGTCGCCATGTGGAATACGCCCCCCAGCTGCAGTTTGCtt < 1:489246/56‑1 (MQ=255) cATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCa < 1:2716958/71‑1 (MQ=255) cATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCa < 1:2608298/71‑1 (MQ=255) tAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCaa < 1:2118744/70‑1 (MQ=255) ggTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACgg < 1:1448004/71‑1 (MQ=255) cAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCAcc > 1:1768981/1‑69 (MQ=255) tcggtcgCCATATGGAATACGCCCCCCAGCTGCAGTTTGCt < 1:2449313/41‑1 (MQ=255) ggtcgCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGa < 1:1774140/71‑1 (MQ=255) ggtcgCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGa > 1:2187962/1‑71 (MQ=255) gtcgCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTg < 1:623323/65‑1 (MQ=255) gtcgCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAAc < 1:2630544/68‑1 (MQ=255) gtcgCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGa < 1:2781716/70‑1 (MQ=255) gtcgCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGa < 1:2072235/70‑1 (MQ=255) gCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATAc < 1:1352824/70‑1 (MQ=255) tatGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTg < 1:2186693/58‑1 (MQ=255) atGGAATACGCCCCGCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGtt > 1:405628/1‑71 (MQ=255) | TTTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGATACGGCGTT > W3110S.gb/3100912‑3101051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |