| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,140,619 | C→T | 12.1% | A343T (GCA→ACA) | hybC ← | hydrogenase 2, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,140,619 | 0 | C | T | 12.1% | 90.9 / 4.3 | 33 | A343T (GCA→ACA) | hybC | hydrogenase 2, large subunit |
| Reads supporting (aligned to +/- strand): ref base C (11/18); new base T (2/2); total (13/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACCCAGGAATATTTCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTA > W3110S.gb/3140551‑3140682 | cACCCAGGAATATTTCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGcc < 1:1138623/70‑1 (MQ=255) cAGGAATATTTCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGTCTgcgg > 1:2621911/1‑71 (MQ=255) tCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTc < 1:1902047/70‑1 (MQ=255) tcgtcAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCtt < 1:162300/67‑1 (MQ=255) gtcAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGTCTGCGGCGCTTCGTCTTta > 1:2697393/1‑67 (MQ=255) gtcAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATAc > 1:514444/1‑70 (MQ=255) aGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAgg < 1:1837343/71‑1 (MQ=255) ccaTCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCtt > 1:2570614/1‑71 (MQ=255) atcatAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCtt < 1:686295/53‑1 (MQ=255) atcatAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCtt < 1:2548656/53‑1 (MQ=255) atcatAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTtata > 1:739229/1‑57 (MQ=255) tcatAAGCCGGAATGGGGGTGCCTTCCCACGGTGCCTGCGgcgc < 1:609974/44‑1 (MQ=255) catAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATAcc > 1:500377/1‑57 (MQ=255) catAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCt < 1:1305228/66‑1 (MQ=255) catAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCt < 1:2089805/66‑1 (MQ=255) atAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCgcgc > 1:23479/1‑71 (MQ=255) ggAATGGTGGTGCCTTCCCACGGTGTCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTg < 1:1198096/71‑1 (MQ=255) ggAATGGTGGTGCCTTCCCACGGTGTCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTg < 1:209655/71‑1 (MQ=255) ggAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTg < 1:82984/71‑1 (MQ=255) ggAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTg < 1:2510255/71‑1 (MQ=255) gAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTg < 1:1655380/70‑1 (MQ=255) aTGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTta < 1:2420427/42‑1 (MQ=255) tggtggTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTc < 1:1449146/56‑1 (MQ=255) tggtggTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATg > 1:50158/1‑71 (MQ=255) gtggtgCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGcc > 1:142857/1‑71 (MQ=255) gtgCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTg < 1:2204964/62‑1 (MQ=255) gtgCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGaa > 1:1522282/1‑64 (MQ=255) tgCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTg < 1:307323/71‑1 (MQ=255) tgCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTg < 1:1087266/71‑1 (MQ=255) ttCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCttt < 1:1599701/53‑1 (MQ=255) ttCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTg < 1:1214801/67‑1 (MQ=255) cccACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGAt > 1:1655925/1‑67 (MQ=255) cACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTa > 1:1820950/1‑71 (MQ=255) ggTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCt > 1:344613/1‑37 (MQ=255) | CACCCAGGAATATTTCCCGTCGTCAGACCAACCATCATAAGCCGGAATGGTGGTGCCTTCCCACGGTGCCTGCGGCGCTTCGTCTTTATACCAGGAGTGCTTCGCGCTTTCCTGAATGCCTTTGATCAGGTA > W3110S.gb/3140551‑3140682 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |