| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,141,409 | C→T | 9.7% | A79A (GCG→GCA) | hybC ← | hydrogenase 2, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,141,409 | 0 | C | T | 9.7% | 183.2 / 8.6 | 62 | A79A (GCG→GCA) | hybC | hydrogenase 2, large subunit |
| Reads supporting (aligned to +/- strand): ref base C (26/30); new base T (4/2); total (30/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01 | |||||||||||
TGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTG > W3110S.gb/3141345‑3141474 | tggtgTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACtt < 1:2277415/71‑1 (MQ=255) gtgCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTg > 1:878322/1‑71 (MQ=255) tgagcAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGc > 1:1175936/4‑71 (MQ=255) tgCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACt < 1:1691310/65‑1 (MQ=255) cgcAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCg > 1:2346571/1‑71 (MQ=255) gCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACg < 1:1372018/71‑1 (MQ=255) aaTGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACg < 1:2185699/66‑1 (MQ=255) aaTGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACg > 1:2543725/1‑70 (MQ=255) aaTGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACg > 1:1540222/1‑70 (MQ=255) atgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg > 1:2143414/1‑70 (MQ=255) atgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg > 1:1829861/1‑70 (MQ=255) atgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg > 1:1755395/1‑70 (MQ=255) atgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg > 1:259719/1‑70 (MQ=255) atgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg > 1:2044723/1‑70 (MQ=255) atgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg > 1:523136/1‑70 (MQ=255) atgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg > 1:1425433/1‑70 (MQ=255) gatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACgg > 1:2054916/1‑68 (MQ=255) atgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAgcg > 1:2329791/1‑46 (MQ=255) atgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGa > 1:1167396/1‑71 (MQ=255) atgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGa > 1:2219261/1‑71 (MQ=255) atgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAAAGGAAGa < 1:214666/71‑1 (MQ=255) atgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTAAgcg > 1:2734827/1‑46 (MQ=255) gTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGa > 1:1743156/1‑69 (MQ=255) ttACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACa < 1:893137/70‑1 (MQ=255) ttACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAg > 1:646873/1‑71 (MQ=255) ttACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAg < 1:25517/71‑1 (MQ=255) ttACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAg < 1:1785036/71‑1 (MQ=255) tACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAgc > 1:1681254/1‑71 (MQ=255) aCGGATGTATTGCGTGTTAACCGGAACGTCGATATTCAGCGCAc > 1:2407583/1‑44 (MQ=255) aCGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGAc > 1:957034/1‑67 (MQ=255) aCGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAgc > 1:1702167/1‑70 (MQ=255) aCGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAgc > 1:577553/1‑70 (MQ=255) gATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCC‑CACGAACGGAAGACAGCGCGTg < 1:974801/71‑1 (MQ=255) aTGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg < 1:345987/58‑1 (MQ=255) aTGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTg < 1:1833583/71‑1 (MQ=255) aTGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTg < 1:2548495/71‑1 (MQ=255) aTGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTg < 1:1239694/71‑1 (MQ=255) aTGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTg < 1:441585/71‑1 (MQ=255) tATTGCGCGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAg < 1:1043926/70‑1 (MQ=255) tATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAg < 1:1228885/70‑1 (MQ=255) ttGCGCGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTgg < 1:1375143/71‑1 (MQ=255) ttGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAAc > 1:1033619/1‑51 (MQ=255) ttGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGaa < 1:2430293/55‑1 (MQ=255) ttGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGt < 1:1651976/69‑1 (MQ=255) ttGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTgg > 1:1264338/1‑71 (MQ=255) ttGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTgg > 1:2710099/1‑71 (MQ=255) gcgcGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGaa > 1:1253142/1‑48 (MQ=255) gcgcGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgt < 1:1031679/70‑1 (MQ=255) gcgcGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgt < 1:2435352/70‑1 (MQ=255) gcgcGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgt < 1:58750/70‑1 (MQ=255) ttAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGt < 1:1247560/58‑1 (MQ=255) ttAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACa < 1:1645459/71‑1 (MQ=255) ttAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACa < 1:1451159/71‑1 (MQ=255) aaCCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATa > 1:1887482/1‑71 (MQ=255) aCGTCGATATTCAGCGCACTTTCTGCCGCACGAAGGGAAGACa > 1:2601491/1‑43 (MQ=255) aCGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACa > 1:2024912/1‑43 (MQ=255) aCGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCcaca < 1:2723328/71‑1 (MQ=255) cGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgtagta < 1:1535637/59‑1 (MQ=255) cGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgtagta < 1:1302210/59‑1 (MQ=255) tCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGt < 1:2613491/46‑1 (MQ=255) ttCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGc < 1:770481/58‑1 (MQ=255) ttCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGc < 1:2125861/58‑1 (MQ=255) ttCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTg < 1:150255/71‑1 (MQ=255) cAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCca > 1:859956/1‑58 (MQ=255) | TGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTG > W3110S.gb/3141345‑3141474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |