| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,204,990 | T→C | 13.0% | intergenic (‑78/‑129) | ygiP ← / → ttdA | predicted DNA‑binding transcriptional regulator/L‑tartrate dehydratase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,204,990 | 0 | T | C | 13.0% | 113.1 / 10.4 | 46 | intergenic (‑78/‑129) | ygiP/ttdA | predicted DNA‑binding transcriptional regulator/L‑tartrate dehydratase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (22/18); new base C (3/3); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 | |||||||||||
AGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACA > W3110S.gb/3204927‑3205058 | aGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTAtt < 1:2722787/71‑1 (MQ=255) aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGt > 1:1498333/1‑71 (MQ=255) aaGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGtt > 1:2325852/1‑70 (MQ=255) aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTTCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGa < 1:2231439/71‑1 (MQ=255) cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGAtt < 1:933337/71‑1 (MQ=255) tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTAtt > 1:1595897/1‑52 (MQ=255) tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACg < 1:1482707/68‑1 (MQ=255) tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAc > 1:2644090/1‑71 (MQ=255) tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAc < 1:1722170/71‑1 (MQ=255) tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAAc > 1:2155104/1‑71 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTg > 1:301782/1‑71 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTg > 1:908238/1‑71 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTg > 1:865628/1‑71 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTg > 1:85865/1‑71 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTg > 1:2406858/1‑71 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTAATTCTTATTTTCGCGTTGATTCACGa > 1:2172173/1‑67 (MQ=255) tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGa > 1:368971/1‑58 (MQ=255) tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGt > 1:1206361/1‑70 (MQ=255) tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGt > 1:498811/1‑70 (MQ=255) tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGt > 1:2106707/1‑70 (MQ=255) cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGtt < 1:2048271/70‑1 (MQ=255) ttGCCATTCCCTGCTCTGGCGCAATATTCATTCTT‑TTTTCGCGTTGATTCACg < 1:2556904/53‑1 (MQ=255) gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATAtt < 1:1876303/70‑1 (MQ=255) gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATAtt > 1:2196694/1‑70 (MQ=255) cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGa < 1:992920/44‑1 (MQ=255) ttCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGAtt < 1:151/44‑1 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACat > 1:715062/1‑61 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGt > 1:868382/1‑70 (MQ=255) cccTGCTCTGGCACAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTg > 1:290864/1‑71 (MQ=255) cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTa < 1:792721/52‑1 (MQ=255) tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGa < 1:1459520/44‑1 (MQ=255) tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACata > 1:67396/1‑59 (MQ=255) tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACata > 1:513704/1‑59 (MQ=255) tGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGc > 1:2264017/1‑70 (MQ=255) ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGtt < 1:874547/48‑1 (MQ=255) ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGtt < 1:1865475/48‑1 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCa > 1:1106331/1‑60 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCa > 1:624985/1‑60 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCaaa > 1:2267665/1‑70 (MQ=255) gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCaaaaaa < 1:1004652/69‑1 (MQ=255) cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATaa < 1:1440100/71‑1 (MQ=255) cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATaa < 1:1186781/71‑1 (MQ=255) cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATaa < 1:1984536/71‑1 (MQ=255) cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATaa < 1:1249220/71‑1 (MQ=255) tatTCATTCTTATTTTCGCGTTGATTCACGAACTGtt < 1:460997/37‑1 (MQ=255) tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTa < 1:728548/42‑1 (MQ=255) ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACgg > 1:1434849/1‑51 (MQ=255) ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACgg > 1:1474040/1‑51 (MQ=255) ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACg > 1:1544605/1‑50 (MQ=255) ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCaaaa < 1:2017964/59‑1 (MQ=255) ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATaa < 1:2512916/66‑1 (MQ=255) ttcactcTTATTTTCGCGTTGATTCACGAACTGTTAATTACata > 1:1148734/1‑44 (MQ=255) cattcTTATTTTCGCGTTGATTCACGAACTGTTaa > 1:2100847/1‑35 (MQ=255) cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACcaca > 1:1360981/1‑71 (MQ=255) cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACca > 1:1203051/1‑69 (MQ=255) | AGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACA > W3110S.gb/3204927‑3205058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |