| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,211,677 | C→T | 9.4% | intergenic (+169/‑26) | dnaG → / → rpoD | DNA primase/RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,211,677 | 0 | C | T | 9.4% | 159.3 / 6.2 | 53 | intergenic (+169/‑26) | dnaG/rpoD | DNA primase/RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base C (24/24); new base T (3/2); total (27/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.68e-01 | |||||||||||
AAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACC > W3110S.gb/3211612‑3211744 | aaaCAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGa > 1:1508158/1‑70 (MQ=255) aacaacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGaaa > 1:1708140/1‑71 (MQ=255) aacaacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGaaa > 1:1620267/1‑71 (MQ=255) acaacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAAt < 1:54494/71‑1 (MQ=255) caacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAAt > 1:504053/1‑70 (MQ=255) ttGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGtgtg > 1:1244920/1‑71 (MQ=255) ttGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGtgtg < 1:345362/71‑1 (MQ=255) tGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAgtg > 1:1152296/1‑68 (MQ=255) tCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCg > 1:2213439/1‑71 (MQ=255) gCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCt < 1:249239/71‑1 (MQ=255) gTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTTATGAAATAAGTGTGGATACCGTCTTa < 1:569545/71‑1 (MQ=255) gTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTTATGAAATAAGTGTGGATACCGTCTTa < 1:566236/71‑1 (MQ=255) tAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGtgtg < 1:1808299/57‑1 (MQ=255) tAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGtgtg < 1:1736702/57‑1 (MQ=255) aaCGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGt > 1:546882/1‑64 (MQ=255) aaCGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATg < 1:920025/70‑1 (MQ=255) cGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCt < 1:2408426/64‑1 (MQ=255) gCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACAGTCTTa > 1:1346145/1‑64 (MQ=255) cTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCaa < 1:1994484/71‑1 (MQ=255) tGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGa > 1:569832/1‑52 (MQ=255) tGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCaa > 1:1759009/1‑70 (MQ=255) aaGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATTAGTGTGGAt < 1:1758941/51‑1 (MQ=255) aaGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGtgtg < 1:2593301/48‑1 (MQ=255) aaGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGAt < 1:95323/51‑1 (MQ=255) aGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAg < 1:1322655/43‑1 (MQ=255) aGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAg < 1:2690238/43‑1 (MQ=255) cATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGtgtg < 1:535495/43‑1 (MQ=255) cATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGtgtg < 1:2006926/43‑1 (MQ=255) cGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGa > 1:1627340/1‑42 (MQ=255) gggTCAATCGCCCAACACCAACCTTATGAAATAAGTGTGGATACCGTCTTATg > 1:2123081/1‑53 (MQ=255) gggTCAATCGCCCAACACCAACCTTATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTca > 1:144186/1‑71 (MQ=255) gTCAATCGCCCAACACCAACCTCATGAAATAAGtgtg < 1:480048/37‑1 (MQ=255) gTCAATCGCCCAACACCAACCTCATGAAATAAGtgtg < 1:456202/37‑1 (MQ=255) tCAATCGCCCAACACCAACCTTATGAAATAAGTGTGGATACCGTCTTATGGAg > 1:1664730/1‑53 (MQ=255) tCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCt < 1:563720/42‑1 (MQ=255) tCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCaaaa < 1:1168255/54‑1 (MQ=255) tCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGa < 1:391166/71‑1 (MQ=255) tccccATCACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGaa < 1:2524589/69‑1 (MQ=255) cGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAc > 1:803961/1‑54 (MQ=255) cGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAc > 1:1602934/1‑54 (MQ=255) cGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAc > 1:1185695/1‑54 (MQ=255) gCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTa > 1:1909482/1‑42 (MQ=255) gCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGaaa > 1:761870/1‑71 (MQ=255) gCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGaaa > 1:2691437/1‑71 (MQ=255) gCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGaaa > 1:1465960/1‑71 (MQ=255) acacCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAActtct > 1:1931270/1‑71 (MQ=255) acacCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAActtct > 1:1255740/1‑71 (MQ=255) cacCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAActtctt < 1:2044136/71‑1 (MQ=255) acCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTg > 1:2543293/1‑71 (MQ=255) ccAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTca > 1:1076772/1‑54 (MQ=255) aaCCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTg > 1:306433/1‑68 (MQ=255) ccTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCAcc < 1:1602029/71‑1 (MQ=255) cTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGa < 1:1022918/56‑1 (MQ=255) cATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGaaa > 1:2152846/1‑56 (MQ=255) cATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGaaa > 1:1862912/1‑56 (MQ=255) | AAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACC > W3110S.gb/3211612‑3211744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |