| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,222,206 | C→T | 78.1% | N306N (AAC→AAT) | ebgA → | cryptic beta‑D‑galactosidase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,222,206 | 0 | C | T | 78.1% | 126.0 / 34.9 | 64 | N306N (AAC→AAT) | ebgA | cryptic beta‑D‑galactosidase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (4/10); new base T (29/21); total (33/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.13e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 | |||||||||||
AATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGG > W3110S.gb/3222142‑3222272 | aaTGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTg > 1:1245572/1‑71 (MQ=255)aaTGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTg > 1:1516366/1‑71 (MQ=255) aTGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTg > 1:183686/1‑70 (MQ=255) ggTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTgg > 1:134553/1‑69 (MQ=255) gTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAg > 1:1298357/1‑71 (MQ=255) gTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAg > 1:2102518/1‑71 (MQ=255) tCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAgt < 1:2371451/71‑1 (MQ=255) tCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAgt < 1:766156/71‑1 (MQ=255) tCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAgt < 1:2571552/71‑1 (MQ=255) tCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAgt < 1:794661/71‑1 (MQ=255) aTCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCcac < 1:260729/71‑1 (MQ=255) aTCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCcac < 1:1918791/71‑1 (MQ=255) cccTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACaa > 1:2283279/1‑70 (MQ=255) cccTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACaa > 1:2662374/1‑70 (MQ=255) cTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAAcgc < 1:1979534/71‑1 (MQ=255) ttATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCcac < 1:1538745/65‑1 (MQ=255) tATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGt > 1:421427/1‑71 (MQ=255) tATCTTTACCATCTGGTCATGACGCTGAAAGACGCAAACGGCAATGTTCTGGAAgtggt > 1:1166943/1‑59 (MQ=255) aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGtt > 1:1977524/1‑71 (MQ=255) aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGtt > 1:2099967/1‑71 (MQ=255) aTCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGtt > 1:897725/1‑71 (MQ=255) tCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTg > 1:851158/1‑71 (MQ=255) tCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTg > 1:865148/1‑71 (MQ=255) cTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAgtggt < 1:582414/56‑1 (MQ=255) cTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAgtgg > 1:356487/1‑55 (MQ=255) cTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTgg > 1:2495174/1‑71 (MQ=255) ccATCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAAcgcg < 1:164489/62‑1 (MQ=255) aTCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt < 1:699609/71‑1 (MQ=255) aTCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt < 1:2789981/71‑1 (MQ=255) aTCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt < 1:2751126/71‑1 (MQ=255) aTCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt < 1:889956/71‑1 (MQ=255) aTCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt < 1:2497897/71‑1 (MQ=255) tCTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGt < 1:2461778/70‑1 (MQ=255) tCTGGTCATGACGCTGAAAGACGCCAACGGCAACGt < 1:1618834/36‑1 (MQ=255) tCTGGTCATGACGCTGAAAGACGCCAACGGCAACGt < 1:766045/36‑1 (MQ=255) tCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAAcgcg < 1:864375/59‑1 (MQ=255) tCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAAcgcg < 1:2597663/59‑1 (MQ=255) cTGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGc < 1:273590/49‑1 (MQ=255) tGGTCATGACGCTGATAGACGCCAACGGCAACGTTc > 1:399004/1‑36 (MQ=255) tGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGa > 1:2395619/1‑70 (MQ=255) tGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGa > 1:1547657/1‑70 (MQ=255) tGGTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGa > 1:2079899/1‑70 (MQ=255) gTCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGatat > 1:2101167/1‑71 (MQ=255) tCATGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAgt < 1:1785399/40‑1 (MQ=255) cATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGt > 1:2359291/1‑54 (MQ=255) aTGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa > 1:418714/1‑70 (MQ=255) aTGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa > 1:2723452/1‑70 (MQ=255) aTGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa > 1:52506/1‑70 (MQ=255) aTGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa < 1:1658140/70‑1 (MQ=255) aTGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa > 1:710876/1‑70 (MQ=255) aTGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCa > 1:232591/1‑70 (MQ=255) aTGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGATGGCTTCCGTGATATCaa < 1:680475/71‑1 (MQ=255) tGACGCTGAAAGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCaa < 1:1280738/70‑1 (MQ=255) tGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAAcgcg < 1:1233277/51‑1 (MQ=255) aCGCTGAAAGACGCCAACGGCAATGTTCTGGAAgtggt > 1:973642/1‑38 (MQ=255) aCGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAg < 1:2201221/70‑1 (MQ=255) aGACGCCAACGGCAATGTTCTGGAAGTTGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACg < 1:1072093/71‑1 (MQ=255) aGACGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACg < 1:1284244/71‑1 (MQ=255) cGCCAACGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGTCTTCCGTGATATCAAAGTGCGCGACGGTc > 1:1274283/1‑71 (MQ=255) aCGGCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTc > 1:810584/1‑71 (MQ=255) ggCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTg > 1:2332969/1‑71 (MQ=255) ggCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTg > 1:2786217/1‑71 (MQ=255) ggCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTg < 1:2323031/71‑1 (MQ=255) gCAATGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTgg < 1:370100/71‑1 (MQ=255) | AATGGTCAGCAGAATCCCCTTATCTTTACCATCTGGTCATGACGCTGAAAGACGCCAACGGCAACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGG > W3110S.gb/3222142‑3222272 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |