| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,232,470 | A→G | 79.0% | L111L (TTG→CTG) | ygjM ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,232,470 | 0 | A | G | 79.0% | 79.0 / 16.5 | 38 | L111L (TTG→CTG) | ygjM | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (6/2); new base G (19/11); total (25/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATC > W3110S.gb/3232402‑3232536 | gggAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCaaa < 1:583397/70‑1 (MQ=255) agaAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAAcgc > 1:1557082/1‑71 (MQ=255) aTGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCagaa > 1:1692065/1‑64 (MQ=255) tGCCGAATCGCGTTGCCAA‑TTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGt < 1:196325/67‑1 (MQ=255) gCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGaca > 1:2411742/1‑71 (MQ=255) gCCGAATCGCGTTGCCAAATTTTTAGGGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGaca > 1:461835/1‑71 (MQ=255) ccGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGacac > 1:370511/1‑71 (MQ=255) ccGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGacac > 1:619189/1‑71 (MQ=255) ccGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGacac > 1:2530991/1‑71 (MQ=255) aaTCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCAt < 1:2445511/71‑1 (MQ=255) aTCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATa < 1:495805/71‑1 (MQ=255) ttGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTa > 1:948199/1‑71 (MQ=255) gCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTa > 1:244175/1‑69 (MQ=255) gCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACt > 1:240189/1‑71 (MQ=255) ttttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGAtt < 1:1991354/62‑1 (MQ=255) ttttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGAtt < 1:2123433/62‑1 (MQ=255) ttttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGAtt < 1:2288672/62‑1 (MQ=255) tttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAAt > 1:1090854/1‑71 (MQ=255) ttttAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAAtt > 1:557967/1‑71 (MQ=255) gtgtTCCAGCGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGa < 1:45086/51‑1 (MQ=255) ttCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTGCGGCAGa < 1:1047115/70‑1 (MQ=255) ttCCAGCGTTAATTTCCTCTTCACGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGa < 1:41332/70‑1 (MQ=255) cGTTAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCgg > 1:841966/1‑60 (MQ=255) cGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACAcc > 1:241835/1‑37 (MQ=255) cGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACAcc > 1:2626731/1‑37 (MQ=255) cGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACAcc > 1:1334234/1‑37 (MQ=255) ttAATTTCCTCTTCCCGCTCAGAACGCGTGACACCATAGAttt < 1:2001719/43‑1 (MQ=255) aaTTTCCTCTTCCCGCTCAGAACGCGTGACACCATa > 1:2750772/1‑36 (MQ=255) tttCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAg < 1:153186/57‑1 (MQ=255) tCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGaaa > 1:2130509/1‑63 (MQ=255) tCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGaaa > 1:1925946/1‑63 (MQ=255) tCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGaaa > 1:990324/1‑63 (MQ=255) tCCTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTaaa > 1:2562329/1‑71 (MQ=255) tCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTaaa > 1:2375166/1‑71 (MQ=255) tCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTaaa > 1:1187456/1‑71 (MQ=255) ccTCTTCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAAc > 1:2088765/1‑71 (MQ=255) ttCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCata > 1:91105/1‑71 (MQ=255) ttCCCGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCa < 1:1058442/69‑1 (MQ=255) cccGCTCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATAtt > 1:585531/1‑71 (MQ=255) tCAGAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATc < 1:1001190/70‑1 (MQ=255) | GGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCGCTCAAAACGCGTGACACCATAGATTTACTGCCAATTTCCGGCAGATCGGAAAGGGTTAAACCATATTGATC > W3110S.gb/3232402‑3232536 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |