| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,272,348 | C→T | 10.4% | G89S (GGT→AGT) | garR ← | tartronate semialdehyde reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,272,348 | 0 | C | T | 10.4% | 138.1 / 6.5 | 48 | G89S (GGT→AGT) | garR | tartronate semialdehyde reductase |
| Reads supporting (aligned to +/- strand): ref base C (24/19); new base T (2/3); total (26/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01 | |||||||||||
GCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGGCAGC > W3110S.gb/3272278‑3272418 | gCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTAcc > 1:33307/1‑71 (MQ=255) ttCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTgg > 1:2489541/1‑71 (MQ=255) gcTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTcgc < 1:2009433/71‑1 (MQ=255) cGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGcc < 1:622753/70‑1 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCaat < 1:2147822/71‑1 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCgat < 1:2675151/71‑1 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaat < 1:2301060/71‑1 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaat < 1:1787627/71‑1 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaa < 1:377655/70‑1 (MQ=255) aTTTCACGGCTTGCCAGCGGCGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaat < 1:1536756/71‑1 (MQ=255) ttCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCaataa > 1:353903/1‑71 (MQ=255) gCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTTGCTTCGCGCCTTCAATAATGCCATtct < 1:1499238/70‑1 (MQ=255) ccAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATtctc < 1:1990964/70‑1 (MQ=255) tGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAgcg > 1:1812085/1‑71 (MQ=255) gATAGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCaat < 1:2664504/48‑1 (MQ=255) aTAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaat < 1:2185648/47‑1 (MQ=255) tAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCtt > 1:506940/1‑42 (MQ=255) aGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaataa > 1:2634520/1‑47 (MQ=255) aGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaataa > 1:213880/1‑47 (MQ=255) aGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaataa > 1:2036779/1‑47 (MQ=255) aGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCa > 1:1063511/1‑52 (MQ=255) aGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCa > 1:2461748/1‑52 (MQ=255) aGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACct > 1:203532/1‑71 (MQ=255) gAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaat < 1:973816/44‑1 (MQ=255) gAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCAtt > 1:1928516/1‑53 (MQ=255) gAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACctc < 1:290809/71‑1 (MQ=255) gAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACct < 1:27670/70‑1 (MQ=255) cTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCaataa > 1:2470216/1‑43 (MQ=255) cTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCa > 1:1700237/1‑64 (MQ=255) cATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACctct > 1:1902407/1‑67 (MQ=255) cATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACctct > 1:1649266/1‑67 (MQ=255) atatCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTca > 1:196869/1‑70 (MQ=255) atatCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTca < 1:1758105/70‑1 (MQ=255) atatCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTca < 1:1078747/70‑1 (MQ=255) tatCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCAtt < 1:1634593/46‑1 (MQ=255) tatCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTca < 1:2184889/69‑1 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCa > 1:631013/1‑48 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt > 1:1616017/1‑70 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt < 1:1998025/70‑1 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt > 1:582778/1‑70 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt > 1:1041515/1‑70 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCCCCACct > 1:1491749/1‑61 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCAc > 1:723998/1‑39 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCAc > 1:2011977/1‑39 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCAc > 1:1646604/1‑39 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCAc > 1:236334/1‑39 (MQ=255) cGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTca < 1:2092365/56‑1 (MQ=255) tACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGgc < 1:247090/71‑1 (MQ=255) aCCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTgg > 1:1940148/1‑69 (MQ=255) ccTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTcac < 1:1351569/53‑1 (MQ=255) ccTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGgcag < 1:638908/71‑1 (MQ=255) cTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAggg < 1:2607018/59‑1 (MQ=255) cTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGgcagc < 1:329650/71‑1 (MQ=255) cTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGgcagc < 1:2092505/71‑1 (MQ=255) | GCTTTCAGCGCTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGGCAGC > W3110S.gb/3272278‑3272418 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |