Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	3,277,106	A→G	70.4%	T84A (ACT→GCT)	sohA →	predicted regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,277,106	0	A	G	70.4%	82.9 / 39.5	54	T84A (ACT→GCT)	sohA	predicted regulator
Reads supporting (aligned to +/- strand):  ref base A (8/8);  new base G (22/16);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 7.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAG  >  W3110S.gb/3277039‑3277174                                                                    |                                                                     ggaggaTCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTc                                                                   <  1:1786089/71‑1 (MQ=255)    ggaTCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTc                                                                <  1:856452/71‑1 (MQ=255)    ggaTCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTc                                                                <  1:1778412/71‑1 (MQ=255)       tCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcat                                                             >  1:2757227/1‑71 (MQ=255)        cATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcatt                                                            >  1:2203187/1‑71 (MQ=255)           aCTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaa                                                         >  1:1410953/1‑71 (MQ=255)               tGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaacat                                                      <  1:364388/70‑1 (MQ=255)               tGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacatt                                                     >  1:1782950/1‑71 (MQ=255)               tGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacat                                                      >  1:2177212/1‑70 (MQ=255)                  aTGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaacattcaa                                                  <  1:138447/71‑1 (MQ=255)                     cATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCCattcaacattcaac                                                 >  1:1285640/1‑69 (MQ=255)                     cATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCCAttcaacattcaaca                                                <  1:1890480/70‑1 (MQ=255)                       tttttGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCcattcaacattca                                                   <  1:2214296/65‑1 (MQ=255)                         tttGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGga                                            >  1:792816/1‑70 (MQ=255)                             agTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTACATTCAACATTCAACAAGgaaagaa                                       <  1:1862875/70‑1 (MQ=255)                              gTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGgaaagaaa                                      <  1:2186786/71‑1 (MQ=255)                              gTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGgaaagaaa                                      <  1:2775326/71‑1 (MQ=255)                               ttttCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaa                                                         <  1:553678/51‑1 (MQ=255)                                 ttCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattcaa                                                         >  1:2343321/1‑49 (MQ=255)                                 ttCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt                                   >  1:2197267/1‑71 (MQ=255)                                 ttCTGGATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACtt                                   >  1:2756067/1‑71 (MQ=255)                                      gATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattca                                                          <  1:1753823/43‑1 (MQ=255)                                      gATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCcattca                                                          <  1:2329994/43‑1 (MQ=255)                                      gATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTc                                <  1:2749060/69‑1 (MQ=255)                                      gATGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTc                                <  1:20000/69‑1 (MQ=255)                                        tGCAGATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTc                                >  1:1495091/1‑67 (MQ=255)                                          cAGATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTgg                           <  1:2447815/70‑1 (MQ=255)                                          cAGATATCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTgg                           <  1:465873/70‑1 (MQ=255)                                            gATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGgaaaga                                        <  1:2786405/55‑1 (MQ=255)                                            gATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGgaaaga                                        <  1:491324/55‑1 (MQ=255)                                            gATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGgaaaga                                        <  1:933771/55‑1 (MQ=255)                                             atatCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATg                       <  1:1259862/71‑1 (MQ=255)                                               atCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGa                     >  1:836735/1‑71 (MQ=255)                                               atCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGa                     >  1:310311/1‑71 (MQ=255)                                                tCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGAc                    >  1:618142/1‑71 (MQ=255)                                                tCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGAc                    >  1:1291294/1‑71 (MQ=255)                                                tCCAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGAc                    >  1:1490204/1‑71 (MQ=255)                                                tCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGgaaagaa                                       <  1:1115780/52‑1 (MQ=255)                                                  cAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGt                  >  1:1170934/1‑71 (MQ=255)                                                  cAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGt                  >  1:2182512/1‑71 (MQ=255)                                                  cAGAACAACCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGt                  >  1:295486/1‑71 (MQ=255)                                                  cAGAACAACCCGCAAAAAGCTAGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGTCATGGACg                   >  1:641529/1‑70 (MQ=255)                                                     aacaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCaa               <  1:1529412/71‑1 (MQ=255)                                                      acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCaa               >  1:277423/1‑70 (MQ=255)                                                      acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCaa               >  1:247048/1‑70 (MQ=255)                                                      acaacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCaa               >  1:839303/1‑70 (MQ=255)                                                       caacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGa                     >  1:2567369/1‑63 (MQ=255)                                                       caacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACa             >  1:2559825/1‑71 (MQ=255)                                                       caacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACa             >  1:252235/1‑71 (MQ=255)                                                       caacCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACa             >  1:1875490/1‑71 (MQ=255)                                                        aacCCGCAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACAt            <  1:1175202/71‑1 (MQ=255)                                                          cccGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACAtt           >  1:360205/1‑70 (MQ=255)                                                              cAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCa                         >  1:2646737/1‑52 (MQ=255)                                                              cAAAAAGCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATtgatga      >  1:2579779/1‑71 (MQ=255)                                                               aaaaagCTCGTCCATTCAACATTCAACAAGgaaagaaa                                      <  1:116806/38‑1 (MQ=255)                                                                 aaagCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATtgatgatga   <  1:1172174/71‑1 (MQ=255)                                                                  aaaCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATgag  <  1:64695/71‑1 (MQ=255)                                                                    |                                                                     GGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAG  >  W3110S.gb/3277039‑3277174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑