| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,291,041 | A→G | 50.9% | L791L (CTA→CTG) | yraJ → | predicted outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,291,041 | 0 | A | G | 50.9% | ‑0.9 / 76.2 | 53 | L791L (CTA→CTG) | yraJ | predicted outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (10/16); new base G (16/11); total (26/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
CCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAAC > W3110S.gb/3290973‑3291108 | cccTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtata < 1:2263041/71‑1 (MQ=255) ccTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtata < 1:2163985/70‑1 (MQ=255) ggCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGGATACTGTAtctctc > 1:2743671/1‑71 (MQ=255) ctTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGa > 1:312313/1‑70 (MQ=255) gCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATc > 1:2085678/1‑71 (MQ=255) cAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATc > 1:1930241/1‑69 (MQ=255) cAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATc > 1:1887882/1‑69 (MQ=255) cAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCGAGTa < 1:2721369/71‑1 (MQ=255) cAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTa < 1:268181/71‑1 (MQ=255) cGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCGAGTaa < 1:2103777/69‑1 (MQ=255) cGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAAt < 1:889010/71‑1 (MQ=255) gatgatACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCGAGTAAAt > 1:178399/1‑70 (MQ=255) atgatACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATc > 1:652076/1‑70 (MQ=255) cGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCGAGTAAATCACAAAGc < 1:1843998/71‑1 (MQ=255) gggCAACAAAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCGAGTAAATCACAAAGCTg < 1:1173269/71‑1 (MQ=255) caacaaAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCGAGTAAAt > 1:2718717/1‑58 (MQ=255) caacaaAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCGAGTAAAt > 1:1151266/1‑58 (MQ=255) caacaaAGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCGAGTAAAt > 1:2715469/1‑58 (MQ=255) acaaAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAg > 1:1219630/1‑51 (MQ=255) acaaAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATca < 1:540204/58‑1 (MQ=255) acaaAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACaaa > 1:467221/1‑62 (MQ=255) caaAGTATCGTCGATGAGGGCGGCATACTGTAtctctc > 1:513284/1‑38 (MQ=255) caaAGTATCGTCGATGAGGGCGGCATACTGTAtctctc > 1:1211216/1‑38 (MQ=255) caaAGTATCGTCGATGAGG‑‑GGCATACTGTAtctctc > 1:661609/1‑36 (MQ=21) aaaGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTa > 1:1805784/1‑71 (MQ=255) aaGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGa > 1:1101128/1‑41 (MQ=255) aaGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTAc > 1:2196083/1‑71 (MQ=255) aGTATCGTCGATGAGGGCGGCATACTGTATCTCTCTgg < 1:704311/38‑1 (MQ=255) tcgtcgATGAGGGCGGCATACTGTATCTCTCTGGGATATCGAGTAAAt < 1:2647064/48‑1 (MQ=255) tcgtcgATGAGGGCGGCATACTGTATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTg < 1:598554/70‑1 (MQ=255) tcgtcgATGAGGGCGGCATACTATATCTCTCTGGGATATCg < 1:2748314/41‑1 (MQ=255) gtcgATGAGGGCGGCATACTGTATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTggg > 1:1058527/1‑70 (MQ=255) gtcgATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTggg > 1:434927/1‑70 (MQ=255) tcgATGAGGGCGGCATACTGTATCTCTCTGGGATATCGAGTAAATcac < 1:2741471/48‑1 (MQ=255) tcgATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACaaa < 1:698578/51‑1 (MQ=255) tcgATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGa < 1:1739386/71‑1 (MQ=255) tcgATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGa < 1:240061/71‑1 (MQ=255) aTGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAAt < 1:2250026/71‑1 (MQ=255) tGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAAt < 1:20025/70‑1 (MQ=255) tGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAAt < 1:1592008/70‑1 (MQ=255) gAGGGCGGCATACTGTATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAAt > 1:422184/1‑69 (MQ=255) aGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAg < 1:2463414/71‑1 (MQ=255) aGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAg > 1:2295139/1‑71 (MQ=255) ggcggcATACTGTATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCa > 1:127495/1‑68 (MQ=255) ggcggcATACTGTATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAgg > 1:1261675/1‑70 (MQ=255) cATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGAta < 1:1178683/71‑2 (MQ=255) tACTGTATCTCTCTGGGATATCGAGTAAATCACAAAGCTg < 1:2684920/40‑1 (MQ=255) tACTGTATCTCTCTGGGATATCGAGTAAATCACAAAGCTg < 1:199343/40‑1 (MQ=255) tACTGTATCTCTCTGGGATATCGAGTAAATCACAAACCTg < 1:2323566/40‑1 (MQ=38) tACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGt < 1:619314/46‑1 (MQ=255) tACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGt < 1:1133973/46‑1 (MQ=255) tACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCa < 1:2632388/61‑1 (MQ=255) cTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGa > 1:2549912/1‑40 (MQ=255) cTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATcaac < 1:2699778/70‑1 (MQ=255) cTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATcaac > 1:1262326/1‑70 (MQ=255) | CCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGATCAAC > W3110S.gb/3290973‑3291108 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |