| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,328,359 | T→C | 14.7% | N71S (AAC→AGC) | greA ← | transcription elongation factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,328,359 | 0 | T | C | 14.7% | 75.0 / 8.1 | 34 | N71S (AAC→AGC) | greA | transcription elongation factor |
| Reads supporting (aligned to +/- strand): ref base T (12/17); new base C (3/2); total (15/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.94e-01 | |||||||||||
GTTACGGTAGCACCAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGTTCAC > W3110S.gb/3328293‑3328416 | gTTACGGTAGCACCAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGCTCGa > 1:2805308/1‑71 (MQ=255) gTAGCACCAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTc < 1:898623/63‑1 (MQ=255) gTAGCACCAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTc < 1:870698/63‑1 (MQ=255) gCACCAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGCTCGACAGCTTGGc > 1:1395862/1‑71 (MQ=255) cAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCg < 1:1003122/71‑1 (MQ=255) aTAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGCTCGACAGCTTGGCTTCGAtctc < 1:1202940/71‑1 (MQ=255) agcgcCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCttt < 1:2346941/69‑1 (MQ=255) gcgcCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTaa < 1:2430975/71‑1 (MQ=255) gcgcCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTaa < 1:1803855/71‑1 (MQ=255) gcCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAAt < 1:673748/70‑1 (MQ=255) aTTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTc < 1:1999038/40‑1 (MQ=255) aTTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTc < 1:238919/40‑1 (MQ=255) ttgttgGGCATTTTGGTGACATCTATCACCTGCGCGCTCGACAGCTTGGCTTCGAt < 1:2639888/56‑1 (MQ=39) ttgttgGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTc < 1:895341/53‑1 (MQ=255) ttgttgGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGAt < 1:443812/56‑1 (MQ=255) ttgttgGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGcc < 1:1550375/71‑1 (MQ=255) gttgGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCtt > 1:1497985/1‑71 (MQ=255) gttgGGCATTTTGGTGACATCAATCACCTGCGCGCTCGACAGCTTGGCTTCGATGt > 1:1029709/1‑56 (MQ=255) gggCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCg < 1:2489809/70‑1 (MQ=255) gCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTc < 1:2629798/46‑1 (MQ=255) ttttGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTaa < 1:971808/54‑1 (MQ=255) ttGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGt > 1:25210/1‑46 (MQ=255) ttGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAAcc > 1:667747/1‑71 (MQ=255) atcaatcaCCTGTGCGTTCGACAGCTTGGCTTCGATGTCt > 1:100163/1‑40 (MQ=255) atcaatcaCCTGCGCGTTCGACAGCTTGGCTTCGATGTCt > 1:2571228/1‑40 (MQ=255) atcaatcaCCTGCGCGTTCGACAGCTTGGCTTCGATGTCt > 1:2018144/1‑40 (MQ=255) atcaatcaCCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTaa > 1:1282690/1‑44 (MQ=255) tcaatcaCCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACg < 1:1298847/47‑1 (MQ=255) aatcaCCTGCGCGTTCGACAGCTTGGCTTCGATGTCttt < 1:2419374/39‑1 (MQ=255) aatcaCCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGTTCAc > 1:398022/1‑71 (MQ=255) aatcaCCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGTTCAc > 1:2264745/1‑71 (MQ=255) aCCTGCGCTTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGaaa > 1:1527082/1‑54 (MQ=255) aCCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGc > 1:597709/1‑43 (MQ=255) aCCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCg > 1:441980/1‑48 (MQ=255) ttCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCa < 1:2641065/41‑1 (MQ=255) | GTTACGGTAGCACCAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGTTCAC > W3110S.gb/3328293‑3328416 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |