| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,351,511 | A→G | 36.1% | V457A (GTG→GCG) | arcB ← | hybrid sensory histidine kinase in two‑component regulatory system with ArcA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,351,511 | 0 | A | G | 36.1% | 29.3 / 34.6 | 36 | V457A (GTG→GCG) | arcB | hybrid sensory histidine kinase in two‑component regulatory system with ArcA |
| Reads supporting (aligned to +/- strand): ref base A (10/13); new base G (9/4); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
GCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCA > W3110S.gb/3351442‑3351577 | gCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCAc < 1:1409895/71‑1 (MQ=255) cgacgaGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGt < 1:1085867/70‑1 (MQ=255) cgacgaGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGt > 1:2207580/1‑70 (MQ=255) gacgaGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTaa < 1:2337893/71‑1 (MQ=255) gacgaGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTaa < 1:1479729/71‑1 (MQ=255) cgaGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATa < 1:1946160/71‑1 (MQ=255) cgaGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATa < 1:846788/71‑1 (MQ=255) gagaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAAt > 1:880333/1‑69 (MQ=255) gagaAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATa > 1:176401/1‑70 (MQ=255) cGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGc > 1:2472592/1‑70 (MQ=255) ccAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCG‑AAAAtttt < 1:36214/71‑1 (MQ=255) ccAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCG‑AAAAttt > 1:22745/1‑70 (MQ=255) ccggtgccggtgGCAGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCG‑AAAATTTTATCCAg > 1:2792102/1‑71 (MQ=255) ccggtgGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCAt < 1:1445508/70‑1 (MQ=255) gtgGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCG‑AAAATTTTAt < 1:78277/58‑1 (MQ=255) gtgGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCATCCTg > 1:2039610/1‑71 (MQ=255) gCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATa < 1:2791654/38‑1 (MQ=255) aGGTTTACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCATc > 1:2212133/1‑63 (MQ=255) tttACCGCCATGACTGTCTTTCGCCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCATCCTGCGGAATGc > 1:1100091/1‑71 (MQ=255) ttACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAAATTTTATCCAGCTCATCCTGCGGAATGc > 1:395170/1‑71 (MQ=255) ttACCGCCATGACTGTCTTTCACCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCa < 1:1205079/57‑1 (MQ=255) ccGCCATGACTGTCTTTCACCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCATCCTGCGGAATGCCGa < 1:348412/70‑1 (MQ=255) tGACTGTCTTTCGCCTGGTAATACATGGCG‑AAAATTTTATCCAg > 1:1081445/1‑44 (MQ=255) aCTGTCTTTCGCCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCATCCTGCGGAATGCCGa > 1:2563620/1‑62 (MQ=255) aCTGTCTTTCACCTGGTAATACATGGCG‑AAAATTTTATc > 1:2033148/1‑39 (MQ=255) aCTGTCTTTCACCTGGTAATACATGGCG‑AAAATTTTATc > 1:1934707/1‑39 (MQ=255) aCTGTCTTTCACCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCagag < 1:1461329/70‑1 (MQ=255) cTGTCTTTCGCCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCATCCTGCGGAATGCCGATAc < 1:1813981/64‑1 (MQ=255) cTGTCTTTCGCCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCATCCTGCGGAATGCCGATAc < 1:380113/64‑1 (MQ=255) cTGTCTTTCACCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTc < 1:965867/71‑1 (MQ=255) tCTTTCACCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTc > 1:126128/1‑68 (MQ=255) tCTTTCACCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTc > 1:630564/1‑71 (MQ=255) tCTTTCACCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTc > 1:2095434/1‑71 (MQ=255) tttCGCCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCa > 1:2509759/1‑71 (MQ=255) tttCGCCTGGTAATACACGGCG‑AAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCa > 1:1998191/1‑71 (MQ=255) tttCACCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCATCCTGCGGAATGcc < 1:1222490/54‑1 (MQ=255) | GCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCG‑AAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCA > W3110S.gb/3351442‑3351577 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |