Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,404,628 A→C 18.6% intergenic (+315/‑663) yhdH → / → accB predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,404,6280AC18.6% 59.7 / 23.2 43intergenic (+315/‑663)yhdH/accBpredicted oxidoreductase, Zn‑dependent and NAD(P)‑binding/acetyl CoA carboxylase, BCCP subunit
Reads supporting (aligned to +/- strand):  ref base A (22/13);  new base C (3/5);  total (25/18)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

GCTACGCTTAGCCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAG  >  W3110S.gb/3404561‑3404689
                                                                   |                                                             
gctacgctTAGCCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAAtt                                                             >  1:2406055/1‑70 (MQ=255)
        tAGCCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAACTTAATCACTc                                                     >  1:1567212/1‑70 (MQ=255)
               ttacttaTTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTa                                              >  1:2052510/1‑70 (MQ=255)
               ttacttaTTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTAt                                             >  1:1367447/1‑71 (MQ=255)
                tacttaTTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAACTTAATCACTCGAACCTAt                                             >  1:2143893/1‑70 (MQ=255)
                 acttaTTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTAtt                                            >  1:2490081/1‑70 (MQ=255)
                  cttaTTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTAttt                                           <  1:785015/70‑1 (MQ=255)
                  cttaTTTCTGGTACCATGGGGTCAATAATCTGATTTTGTTTGACTACAACTTAATCACTCGAACCTAttt                                           <  1:845461/70‑1 (MQ=255)
                    taTTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTaa                                         >  1:1180737/1‑70 (MQ=255)
                     aTTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTaa                                         <  1:1537833/69‑1 (MQ=255)
                     aTTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTaa                                         <  1:1218426/69‑1 (MQ=255)
                     aTTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATg                                       >  1:2302682/1‑71 (MQ=255)
                      tttCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTaa                                         <  1:1768475/68‑1 (MQ=255)
                            gTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGc                                 <  1:2064120/70‑1 (MQ=255)
                            gTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCa                                <  1:318278/71‑1 (MQ=255)
                             tACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTaa                                         <  1:2744432/61‑1 (MQ=255)
                             tACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTTAGCAt                               >  1:24842/1‑71 (MQ=255)
                              aCCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCAt                               >  1:2213498/1‑70 (MQ=255)
                               ccATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTg                             >  1:2739741/1‑71 (MQ=255)
                                 aTGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGt                            >  1:44494/1‑70 (MQ=255)
                                      gTGAATAATCTGATTTTGTTTGACTACAAaataat                                                          >  1:2470448/1‑35 (MQ=255)
                                      gTGAATAATCTGATTTTGTTTGACTACAAATTAAt                                                          >  1:1925496/1‑35 (MQ=255)
                                       tGAATAATCTGATTTTGTTTGACTACAACTTAATCACTCGAACCTATTTAATGCTGAg                                  <  1:1325314/58‑1 (MQ=255)
                                        gAATAATCTGATTTTGTTTGACTACAACTTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGt                    >  1:1334807/1‑71 (MQ=255)
                                         aataatCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTa                                              >  1:838160/1‑44 (MQ=255)
                                         aataatCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATc                       >  1:97079/1‑67 (MQ=255)
                                         aataatCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGt                    >  1:412778/1‑70 (MQ=255)
                                           taatCTGATTTTGTTTGACTACAACTTAATCACTCGAACCTATTTAATGCTGAGc                                 <  1:908310/55‑1 (MQ=255)
                                           taatCTGATTTTGTTTGACTACAACTTAATCACTCGAACCTATTTAATGCTGAGc                                 <  1:1252490/55‑1 (MQ=255)
                                           taatCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAg                                  <  1:1102300/54‑1 (MQ=255)
                                           taatCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTaa                 >  1:2577827/1‑71 (MQ=255)
                                           taatCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTCAGCAtt                              <  1:1326676/58‑1 (MQ=255)
                                             atCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTAt                                             >  1:586268/1‑41 (MQ=255)
                                              tCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGc                                      <  1:952294/47‑1 (MQ=255)
                                                tGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTg                                    >  1:1372203/1‑47 (MQ=255)
                                                   ttttGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCt                                     <  1:283573/43‑1 (MQ=255)
                                                     ttgtttgACTACAAATTAATCACTCGAACCTATTTaa                                         >  1:2572272/1‑37 (MQ=255)
                                                     ttgtttgACTACAAATTAATCACTCGAACCTATTTaa                                         >  1:753268/1‑37 (MQ=255)
                                                     ttgtttgACTACAAATTAATCACTCGAACCTATTTaa                                         >  1:2131150/1‑37 (MQ=255)
                                                      tgtttgACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCa                                >  1:1409967/1‑45 (MQ=255)
                                                      tgtttgACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCt      >  1:1622709/1‑71 (MQ=255)
                                                           gACTACAACTTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAg  <  1:2628270/70‑1 (MQ=255)
                                                           gACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAg  <  1:2594612/70‑1 (MQ=255)
                                                           gACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAg  <  1:1084344/70‑1 (MQ=255)
                                                                   |                                                             
GCTACGCTTAGCCCCTTACTTATTTCTGGTACCATGGGGTGAATAATCTGATTTTGTTTGACTACAAATTAATCACTCGAACCTATTTAATGCTGAGCATTGTCAATCGGTTAATTTTGCGTGCTTTAG  >  W3110S.gb/3404561‑3404689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: